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Wednesday, March 27

The Electro-Optical Detection of Thrombin

Ivana Karpisova and Michal Pelach, Comenius University, Bratislava, Slovakia
Center for Nanophase Materials Sciences Seminar
11:00 AM — 12:00 PM, Center for Nanophase Materials Sciences
(Building 8610), Room L-183
Contact: Ilia Ivanov (ivanovin@ornl.gov), 865.771.6213

Abstract

Detection of disease-related biomolecules in body fluids such as serum or plasma is expected to have significant impact in clinical diagnostics. Taking advantage of high specificity of some intermolecular recognition reactions, such as antibody-antigen interaction, protein-aptamer recognition it is possible to directly detect disease-related biomolecules without pre-extracting them from the matrix, simplifying the assay procedures notably. Aptamer-protein complex contains of thrombin binding aptamer (TBA) and thrombin protein. Thrombin is a serine protease that in humans is responsible for reduction of blood loss as main coagulator factor but as well could cause various diseases. Coupling of TBA to different transduction principles has demonstrated the wide applicability of aptamers as bio receptors in bio analytical assays.

Proteins adsorbed onto surface bound aptamers can be measured by quartz crystal microbalance, what is also indicated by preliminary results obtained at the Comenius University in Bratislava.

The goal of this project is to critically evaluate all the parameters that can influence the sensor performances by using the thrombin aptamer immobilized onto gold surface of piezoelectric quartz crystals and amplifying detection limit using surface enhanced raman effect (enabled by gold nano particles).