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Human Genome News Archive Edition

Human Genome News, Nov. 1994; 6(4):2

Columbia University (CU) College of Physicians and Surgeons

COLUMBIA UNIVERSITY (CU) COLLEGE OF PHYSICIANS AND SURGEONS
(NIH, established 1993)
ARGIRIS EFSTRATIADIS, Director
Isidore Edelman, Administrative Director
CONTACTS: Edelman (212/305-3440, Fax: -1191; edelman@cuccfa.ccc.columbia.edu) or Efstratiadis (-5773, Fax: /923-2090, arg@cuccfa.ccc.columbia.edu); CU; 701 W. 168th St.; New York, NY 10032.
OTHER KEY RESEARCHERS
Philip Bourne
Eftihia Cayanis
Stuart G. Fischer
Rodney Rothstein
James Russo
Eric Schon
M. Bento Soares
Dorothy Warburton
Peisen Zhang

MAJOR GOALS

  • Construction of a high-resolution physical map of human chromosome 13, consisting of ordered cosmid contigs aligned to yeast artificial chromosomes, annotated with sequence tagged sites at 100-kb average intervals and with cytogenetic assignment of contig members by in situ hybridization.
  • Selection and cytogenetic assignment of cDNAs representing about 20% of the total estimated chromosome 13 genes.
  • Development and integration of gene-based sequence tagged sites into the physical map.

MAJOR ACCOMPLISHMENTS

  • Coverage of an estimated 50% of chromosome 13 with over 500 cosmid contigs aligned to more than 200 yeast artificial chromosomes; development of 115 sequence tagged sites.
  • Identification of over 90 cDNAs hybridizing to single-copy sequences of chromosome 13 and development of corresponding sequence tagged sites.
  • Localization of 265 markers (yeast artificial chromosomes, cosmids, cDNAs, and half-linking clones) to cytogenetic bands of chromosome 13 by in situ hybridization.
  • Development and genetic mapping of 24 polymorphic sequence tagged sites spanning chromosome 13.
  • Contribution to Wilson's disease gene identification.
  • Development of relational database and software (integrated mapping package) supporting physical mapping from yeast artificial chromosome and cosmid cross-hybridization relationships and sequence tagged site data.

AVAILABLE RESOURCES

  • Total human yeast artificial chromosome libraries (Centre d'Etude du Polymorphisme Humain midi- and mega-yeast artificial chromosomes).
  • Arrayed chromosome 13 midi- and mega-yeast artificial chromosome sublibraries (1431 and 827 colonies, respectively; replicate filters and polymerase chain reaction screening pools are available).
  • Arrayed chromosome 13 cosmid library (LANL; 16,996 colonies; replicate filters are available).
  • Yeast artificial chromosome and cosmid screening service for chromosome 13 probes.
  • Normalized cDNA libraries.
  • Integrated mapping package software.
  • Chromosome 13 somatic cell hybrid mapping panel and deletion cell lines.
    HGMIS staff

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Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.