Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):4
Lawrence Berkeley Laboratory (LBL)
LAWRENCE BERKELEY LABORATORY (LBL)
- (DOE, established 1988)
- MOHANDAS NARLA, Director
- CONTACTS: Narla (510/486-4251, Fax: -6746, mohandas_narla@macmail.lbl.gov) or Jennifer Knox, Executive Assistant to Center Director (JLKnox@lbl.gov); LBL; Human Genome Center; 74-157, 1 Cyclotron Road; Berkeley, CA 94720.
OTHER KEY RESEARCHERS
- Jan-Fang Cheng
- Joseph Jaklevic
- William Kimmerly
- William Kolbe
- John McCarthy
- Victor Markowitz
- Christopher Martin
- Michael Palazzolo
- Martin Pollard
- Edward Rubin
- Ed Theil
- Manfred Zorn
MAJOR GOALS
- Development and implementation of directed methodologies, automation, and informatics tools for cost-effective and accurate high-throughput human DNA sequencing. Data management and distribution through collaborations among biologists, the automation group, and computer scientists.
- Assembly of physical maps of complete chromosome arms rooted in templates appropriate for sequencing and development of capacity to produce genomic sequence of multiple megabases per year. One target is the chromosome 5q31 region.
- Discovery of novel human genes in targeted sequencing regions by analyzing mouse phenotypes created through alteration of genetic content of syntenic regions.
MAJOR ACCOMPLISHMENTS
- Completion of 1.5 Mb of sequence, with more than 1 Mb already deposited in public databases.
- Directed strategy is roughly comparable for both invertebrate and mammalian templates, with 600 to 700 kb/yr generated.
- Completion of automation modules, including an image station that captures and analyzes mapping information from agarose gels, a colony picker, a robotic library replicator, and a modified Biomek that sets up polymerase chain reaction assays and sequencing reactions. A water-based thermocycler and a 12-channel oligonucleotide synthesizer are now in testing stages.
- Development of software, including mapping tools for generating distance-orientation-gene-size resolution (DOG) tag and transposon maps, database management tools, enhancements and new data models for ACEDB, software tools for sequencing and marker submission, and automatic logging and report generators for project management.
- Construction of 1.2 Mb P1 map in the interleukin gene cluster region of human chromosome 5q.
- Construction of 3-Mb P1 map in the Down syndrome region of human chromosome 21.
- Construction of in vivo transgenic mice library containing 2 Mb of human sequences from the Down syndrome region.
- Development of transgenic mice containing nested deletions in the syntenic region of human chromosome 5q31.
AVAILABLE RESOURCES
- Automated colony picker and imaging station with supporting hardware for gel casting (M. Pollard, 510/486-4561).
- Databases for representing and displaying genomic data (E. Theil, -7501).
- Biological resources: mapped yeast artificial chromosomes, P1s, and cDNAs in the Down syndrome region of chromosome 21; mapped yeast artificial chromosomes and P1s in the interleukin gene cluster region of chromosome 5; in vivo transgenic mice library containing 2 Mb of human DNA from the Down syndrome region (for transgenic mice library: E. Rubin, -5072, Cheng, -6590).
HGMIS staff
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).
