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Human Genome News Archive Edition

Human Genome News, Nov. 1994; 6(4):6

Stanford Human Genome Center

STANFORD HUMAN GENOME CENTER (SHGC)
(NIH, established 1990)
RICHARD M. MYERS, Director
David R. Cox and Douglas Vollrath, Co-Directors
CONTACTS: Myers (415/725-9687, Fax: -9689; rmyers@camis.stanford.edu), Cox (-8042, Fax: -8058, cox@camis.stanford.edu), Vollrath (/723-3290, Fax: -7016, vollrath@genome.stanford.edu), or Cristina Estébanez, Administration (/812-1915, Fax: -1916, cxe@camis.stanford.edu); SHGC; Dept. of Genetics; 855 California Ave.; Palo Alto, CA 94304.
OTHER KEY RESEARCHERS
Lane Conn
Sid Cowles
Jian-Bing Fan
Richard Goold
Cynthia Keleher
Kathleen McKusick
Christopher Mader
John Quackenbush
Elizabeth Stewart
Laura Stuve

MAJOR GOALS

  • Generation of 1000 sequence tagged sites and high-resolution radiation hybrid and yeast artificial chromosome sequence tagged site-content maps for human chromosome 4.
  • Generation of 30,000 sequence tagged sites throughout the human genome.
  • Construction of two radiation hybrid maps of the entire human genome with average resolutions of 500 kb (6500 sequence tagged sites) and 100 kb (30,000 sequence tagged sites) by 1995 and 1998, respectively.
  • Development and application of technologies for rapid sequencing of human genomic DNA.
  • Development of informatics resources for analyzing and managing human genome mapping data and for sequencing data generated in the center.
  • Development of an integrated education program that includes teacher education, outreach efforts, and genome science curriculum development.

MAJOR ACCOMPLISHMENTS

  • Generation of 1300 sequence tagged sites from human chromosome 4, including 170 meiotically mapped SSRs and 50 known genes. Polymerase chain reaction used to localize most sequence tagged sites within 9 bins.
  • Generation of an additional 3500 sequence tagged sites distributed throughout the human genome and determination of their chromosomal assignment using a somatic cell hybrid panel. Sources for sequence tagged site sequences include Genethon and Univ. of Iowa CHLC microsatellite repeat markers.
  • Isolation of yeast artificial chromosomes from Centre d'Etude du Polymorphisme Humain Mark I and mega-yeast artificial chromosome libraries for 50 Mb of chromosome 4p (600 yeast artificial chromosomes with 389 sequence tagged sites; 650-kb average yeast artificial chromosome insert size) and 150 Mb of chromosome 4q (2700 yeast artificial chromosomes with 900 sequence tagged sites; 800-kb average yeast artificial chromosome insert size). Generation of an sequence tagged site-content map of 4p (155-kb average resolution) from these sequence tagged sites and yeast artificial chromosomes; 318 of 389 sequence tagged sites were uniquely ordered.
  • Construction of a radiation hybrid map of chromosome 4 that includes 689 sequence tagged sites, providing a comprehensive map at a resolution of 0.5 Mb and a framework map at a resolution of 1.0 Mb.
  • Construction of a panel of 86 radiation hybrid cell lines for whole-genome mapping and construction from these hybrids of a map containing the same sequence tagged site map order as that produced with chromosome 4þonly set of radiation hybrids.
  • Establishment of a relational database, computational tools for automatic radiation hybrid map construction and yeast artificial chromosome sequence tagged site-content map generation, and an ftp server containing much of the center's data (shgc.stanford.edu).

AVAILABLE RESOURCES

  • Oligonucleotide sequences and complete sequence tagged site sequences with polymerase chain reaction conditions for over 1300 chromosome 4 sequence tagged sites.
  • Oligonucleotide sequences, complete sequence tagged site sequences with polymerase chain reaction conditions, and chromosomal assignments for 3500 sequence tagged sites distributed around the rest of the human genome.
  • Yeast artificial chromosome clones mapped by sequence tagged site content for 1200 chromosome 4 markers (Research Genetics, Inc., 800/533-4363).
  • Set of 86 radiation hybrids (containing portions of the entire human genome) that allows markers to be mapped at about 500-kb resolution (Research Genetics).
  • Educational resources, including tours of the center; lectures on genome science and human genetics for teachers, students, and lay groups; and information on genome science curricula development.

HGMIS staff

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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.