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Human Genome News Archive Edition

Human Genome News, Nov. 1994; 6(4):8

University of California, Berkeley, Drosophila Genome Center

UNIVERSITY OF CALIFORNIA, BERKELEY (UCB), DROSOPHILA GENOME CENTER
(NIH, established 1992)
GERALD M. RUBIN, Director
CONTACT: Rubin (510/643-9945, Fax: -9947); 539 LSA Bldg.; UCB; Berkeley, CA 94720-3200.
OTHER KEY RESEARCHERS
Daniel L. Hartl (Harvard Univ.)
William Kimmerly (LBL)
Suzanna Lewis
Christopher H. Martin (LBL)
Michael J. Palazzolo (LBL)
Allan C. Spradling (Carnegie Institute of Washington, Howard Hughes Medical Institute)

MAJOR GOALS

  • Generation of a physical map of the Drosophila melanogaster genome by sequence tagged site-content mapping of a P1 library to serve as templates for DNA sequencing.
  • Integration of sites of high biological interest into the physical map, including known genes, cDNAs, and lethal P-element insertion sites.
  • Sequencing of the 120-Mb euchromatic portion of the Drosophila genome.

MAJOR ACCOMPLISHMENTS

  • Preparation of robotically arrayed 10,000-member bacteriophage P1 library for automated screening and contig assembly.
  • Construction of a map by in situ hybridization against polytene chromosomes for over 2500 P1 clones (about 1.5 hits) that are being used as starting points for contig assembly.
  • Mapping of over 1500 sequence tagged sites to the P1 library.
  • Mapping by in situ hybridization of over 2000 P-transposable-element insertion sites.
  • Completion of over 1 Mb of Drosophila genomic sequence.

AVAILABLE RESOURCES

  • Mapped P1 clones that are directly available from 16 different sites.
  • Drosophila strains with mapped single P-element insertions (Indiana Drosophila Stock Center).
  • Adaptation of C. elegans database (ACEDB) to manage and graphically display Drosophila data.
  • Primer information and complete sequence tagged site sequence (average, 300 bp) with polymerase chain reaction conditions of over 1500 sequence tagged sites of known chromosomal position.

HGMIS staff

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Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).

Human Genome Project 1990–2003

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