Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):8
University of California, Berkeley, Drosophila Genome Center
- UNIVERSITY OF CALIFORNIA, BERKELEY (UCB), DROSOPHILA GENOME CENTER
- (NIH, established 1992)
- GERALD M. RUBIN, Director
- CONTACT: Rubin (510/643-9945, Fax: -9947); 539 LSA Bldg.; UCB; Berkeley, CA 94720-3200.
- OTHER KEY RESEARCHERS
- Daniel L. Hartl (Harvard Univ.)
- William Kimmerly (LBL)
- Suzanna Lewis
- Christopher H. Martin (LBL)
- Michael J. Palazzolo (LBL)
- Allan C. Spradling (Carnegie Institute of Washington, Howard Hughes Medical Institute)
- Generation of a physical map of the Drosophila melanogaster genome by sequence tagged site-content mapping of a P1 library to serve as templates for DNA sequencing.
- Integration of sites of high biological interest into the physical map, including known genes, cDNAs, and lethal P-element insertion sites.
- Sequencing of the 120-Mb euchromatic portion of the Drosophila genome.
- Preparation of robotically arrayed 10,000-member bacteriophage P1 library for automated screening and contig assembly.
- Construction of a map by in situ hybridization against polytene chromosomes for over 2500 P1 clones (about 1.5 hits) that are being used as starting points for contig assembly.
- Mapping of over 1500 sequence tagged sites to the P1 library.
- Mapping by in situ hybridization of over 2000 P-transposable-element insertion sites.
- Completion of over 1 Mb of Drosophila genomic sequence.
- Mapped P1 clones that are directly available from 16 different sites.
- Drosophila strains with mapped single P-element insertions (Indiana Drosophila Stock Center).
- Adaptation of C. elegans database (ACEDB) to manage and graphically display Drosophila data.
- Primer information and complete sequence tagged site sequence (average, 300 bp) with polymerase chain reaction conditions of over 1500 sequence tagged sites of known chromosomal position.
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).