Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):8
University of Michigan Medical Center
UNIVERSITY OF MICHIGAN MEDICAL CENTER (UMMC)
- (NIH, established 1990)
- MIRIAM H. MEISLER, Director
- CONTACT: Meisler (313/763-5546, Fax: -9691; miriam.meisler@med.umich.edu); UMMC; Ann Arbor, MI 48109-0618.
OTHER KEY RESEARCHERS
- Diane Baker
- Mike Boehnke
- David Burke
- Sally Camper
- Jeff Chamberlain
- Thomas Glover
- Jerry Gorski
- Sun-Wei Guo
- Thomas Gelehrter
- David Ginsburg
- David Law
- Dorene Markel
- Jerry Slightom (Upjohn Company)
- Spencer Thomas
MAJOR GOALS
- Study of genetic diseases through development and use of novel technologies.
- Advancement and development of genomic technology, especially development of microsatellite markers, fluorescence in situ hybridization mapping, radiation hybrid mapping, genetic mapping, yeast artificial chromosome technology, and DNA sequencing.
- Comparative human-mouse mapping and gene identification with focus on human 17q and mouse chromosome 11. High-resolution genetic and physical mapping of both species.
MAJOR ACCOMPLISHMENTS
- Identification of families and individuals with genetic diseases, resulting in collection of 550 blood samples, immortalization of 500 cell lines, and investigation of over 200 families with breast cancer.
- Generation of new microsatellite repeat polymorphisms, including 20 dinucleotide and more than 80 tetranucleotide markers. The latter are from chromosome 17, and many map near the breast cancer locus on 17q.
- Development of algorithms and software for radiation hybrid mapping, fluorescence in situ hybridization mapping, and estimation of allele frequency based on pedigree data.
- Construction of radiation hybrid panels and characterization of four somatic cell hybrid lines for the long arm of chromosome 17; development of large-scale fluorescence in situ hybridization mapping of yeast artificial chromosome and cosmid clones.
- Direct cycled sequencing of polymerase chain reaction products and direct sequence analysis of CA repeats.
- Screening of 5 to 10 sequence tagged site markers against 4 yeast artificial chromosome libraries each month to complete the chromosome 17 physical map. Production of 5 different cDNA libraries.
- Development of methodology in which microdissected material is labeled by polymerase chain reaction and mapped back to metaphase chromosomes.
AVAILABLE RESOURCES
- cDNA libraries from human retinal pigment epithelium, retina, kidney, fetal brain, and whole fetus.
- Microdissection technology and protocols.
- Chromosome 17 radiation hybrid panel.
- Four chromosome 17q somatic cell hybrids.
- Tetranucleotide and dinucleotide markers from chromosome 17.
- Chromosome 17q yeast artificial chromosomes. (The center serves as a community screening resource.)
- Human and mouse P1 libraries.
- Mouse interspecific backcross; 550 animals typed for chromosome 11 markers.
- Radiation hybrid mapping software (RHMAP).
- Educational resources for clinicians, genetic counselors, journalists, and high school teachers.
HGMIS staff
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).
