Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):10
University of Texas Southwestern Medical Center at Dallas
- UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER AT DALLAS (UTSMCD)
- (NIH, established 1990 at Salk Institute; relocated 1994)
- GLEN A. EVANS, Director
- Harold R. Garner, Associate Center Director
- CONTACTS: Evans (214/648-1660, email@example.com), Suzie Hayes, Administrative Services Officer (-1636), or Garner (-1661, firstname.lastname@example.org); (Center Fax: -1666); UTSMCD; 6000 Harry Hines Blvd.; Dallas, TX 75235-8591.
- OTHER KEY RESEARCHERS
- Anne Bowcock
- David Burbee
- Kim Jackson
- Michael Lovett
- Shane Probst
- Lori Romberg
- Roger Schultz
- Ron Scott
- Sylvia Thomas
- Development of a complete, contiguous, error-free physical map of human chromosome 11 at 100- to 200-kb resolution, based on sequence tagged site-content mapping and nonchimeric yeast artificial chromosome contigs.
- Development of a high-resolution (1- to 5-kb) physical map of human chromosome 11 and selected other chromosomes based on genomic sequence sampling and contig ordering using direct-visualization fluorescence in situ hybridization.
- Determination of the one-pass DNA sequence of 30% of chromosome 11.
- Development of tools, instrumentation, and infrastructure for high-throughput automated DNA sequencing of human chromosome 11 and selected other regions of the human genome by parallel primer walking.
- Development of informatics support, biocomputational tools, and infrastructure for high-throughput automated DNA sequencing of the human genome.
- Development of additional new methods, technologies, and instrumentation for automated mapping and sequencing, including nanovolume sample processing and handling on DNA chips.
- Production of resources necessary for rapid identification and functional characterization of biologically and medically important genes, including cDNA isolation and genotyping.
- Preparation of a collection of over 700 standardized sequence tagged sites, including random sequences mapped by somatic cell hybrid analysis or fluorescence in situ hybridization.
- Isolation of over 900 yeast artificial chromosome clones (>1 Mb) and over 1000 chromosome 11-specific, nonchimeric yeast artificial chromosome clones regionally mapped to chromosome 11.
- Development of a yeast artificial chromosome clone contig map of chromosome 11 with continuous coverage of 80% of the chromosome.
- Development and application of directed end-cloning strategies to produce additional sequence tagged sites from the contig ends for gap filling.
- Development of a novel and potentially rapid strategy for producing high-resolution physical maps and sequencing templates using parallel sequencing and clone fingerprinting (denoted genomic sequence sampling).
- Development of two useful robotic systems, Prepper and GAS (Genome Automation System) for high-throughput sample processing.
- Began development of advanced technology for nanovolume sample processing (Garner) and advanced sequencing using DNA microdevices and chips [in collaboration with Michael Heller (Nanogen, Inc., San Diego)].
- Identification of the site of a novel suppressor oncogene active in cervical carcinoma at the distal end of chromosome 11.
- Cosmids: Arrayed chromosome 11-specific libraries cSRL, 16,000 clones; c11q (11q13-11qter), 1200 clones. cCLM Giardia cosmid libraries, 12,000 clones, 2 hosts.
- Yeast artificial chromosomes: Total genome libraries (St. Louis, Centre d'Etude du Polymorphisme Humain Mark I, Centre d'Etude du Polymorphisme Humain Mark VI to VII mega-base, Centre d'Etude du Polymorphisme Humain/Genethon "mega-yeast artificial chromosome"subset). Chromosome 11-specific library (T. Shows, RPMI, Buffalo, NY).
- Sequence tagged sites: Over 700 sequence tagged sites produced by this lab and >1000 sequence tagged site for chromosome 11 (available online via Internet).
- Cell hybrids: 15 cell hybrid chromosome 11 mapping panels; monochromosomal hybrids for all human chromosomes.
- Instrumentation: Prepper, GAS, GIST (Genome Informatics System on Trans-puters), polymerase chain reaction system, Hyb system (Garner).
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).