Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News Archive Edition
Human Genome Quarterly, Summer 1989; 1(2)
Composite Maps Completed with Data from Several
Scientists engaged in mapping human chromosome 16 met at the Yale University School of Medicine in New Haven, Connecticut, on June 8-9, 1989. The workshop was jointly supported by the U.S. Department of Energy and the National Institutes of Health and was convened by Stephen Reeders. The purpose of the meeting was to integrate information from multiple levels of gene mapping and to construct an up-to-date series of chromosome maps based on various genetic and physical mapping techniques. The 28 attendees represented 16 laboratories from 7 countries.
Chromosome 16 mapping is proceeding at several levels. The following topics, listed here after their respective researcher(s), were included in workshop discussions:
Tim Keith (Collaborative Research, Waltham, Massachusetts) and Cecile Julier (CEPH - Centre d'Étude du Polymorphisme Humain, Paris, France): Genetic maps constructed using reference families - Over 80 polymorphic loci have been mapped using reference families, with loci spaced at average intervals of ~2.5 cM (in the 195-cM+ female map).
Peter O'Connell (HHMI - Howard Hughes Medical Institute, Salt Lake City, Utah): Automated techniques - O'Connell uses automated techniques to overcome sample handling errors, which are a major source of mistakes in genotypes and a major limitation in high-resolution genetic mapping.
Peter Lichter (Yale University, New Haven, Conn.): High-resolution in-situ hybridization - New techniques for multicolor fluorescence in-situ hybridization on metaphase chromosomes developed in David Ward's laboratory are capable of resolving probes spaced approximately 1 Mb apart.
Grant Sutherland (Adelaide Children's Hospital, North Adelaide, Australia): Chromosome break points - A series of chromosome break points effectively divides the chromosome into 25 intervals for rapid physical mapping of new clones.
Giovanni Romeo (Laboratorio di Genetica Molecolare, Istituto Giannina Gaslini, Genoa, Italy): Radiation fragmentation - The use of radiation fragmentation hybrids adds significantly more power to physical mapping by hybrid analysis.
Paul Liu (The University of Texas MD Anderson Cancer Center, Houston, Tex.): Selectable markers for enhanced mapping resolution - CHO hybrids retaining human ERCC4 activity have allowed Liu to create a high-resolution map of the 16p13 region.
Anne-Marie Frischauf (ICRF - Imperial Cancer Research Fund, London, U.K.): Radiation fragmentation hybrids - Her efforts focus on selecting genes from a region by cloning Not I sites from radiation fragmentation hybrids.
Stephen Reeders, Gerry Gillespie, and Greg Germino (Yale University, New Haven, Conn.), Peter Harris (John Radcliffe Hospital, Oxford, U.K.), and Martijn Breuning (State University of Leiden, Leiden, the Netherlands): Pulsed-field-gel mapping - Their work includes macrorestriction mapping in the region of the polycystic kidney disease locus.
Michael Siciliano (The University of Texas MD Anderson Cancer Center, Houston, Tex.): Somatic cell hybrid - He employs a novel technique to isolate human expressed sequences from a somatic cell hybrid.
Ray Stallings (Los Alamos National Laboratory, Los Alamos, N.M.): Overlapping cosmids - He is attempting to construct an array of overlapping cosmids for chromosome 16 starting with flow-sorted chromosomes.
Tom Marr (Los Alamos National Laboratory, Los Alamos, N.M.): Difficulties of handling data from multiple levels of mapping - He is working to develop an automated system with algorithms for detecting cosmid overlaps.
Participants divided into groups to complete composite maps based on the data of several laboratories.
Some of the maps will be published in the report of the Chromosome 16 Committee (co-chaired by Stephen Reeders and Ed Hildebrand, Los Alamos National Laboratory, Los Alamos, N.M.) of the Tenth International Human Gene Mapping Workshop.
Chromosome 16 Workshop maps may be obtained from:
The electronic form of the newsletter may be cited in the
Human Genome Program, U.S. Department of Energy, Human Genome News (v1n2).
The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.
Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.