Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News Archive Edition
Human Genome News, July 1990; 2(2)
Participants Generate Consenses Maps
Laboratory representatives described their research and generated consensus maps of chromosome 3 at a meeting sponsored by NIH and the RGK Foundation. Held in San Antonio at the University of Texas Health Science Center (UTHSC) on February 16-17, the chromosome-3 meeting attracted 42 participants from laboratories in Canada, England, Japan, the Netherlands, Sweden, and the United States. Highlights of some of the papers are included below.
David Smith (Wayne State University) disclosed the isolation of a large number of chromosome-3-specific cosmids and the identification and mapping of several "cluster" cosmids containing multiple rare restriction sites. Harry Drabkin [Eleanor Roosevelt Institute for Cancer Research (ERICR)] reported on a number of markers and the establishment of a radiation hybrid panel. Lakshmi Atchison (Fox Chase Cancer Center) described several projects, including rare-site linking libraries, isolation of markers, mapping by in situ hybridization, and construction of a radiation hybrid panel. Pamela Rabbitts [Medical Research Council (MRC) Cambridge, U.K.] has mapped several markers by in situ hybridization and firmly established reference points; she has also determined that ErbAß is the same locus as ErbA2.
Michael Lerman [National Cancer Institute (NCI), Frederick Cancer Research Facility (FCRF)] reported on 2000 single-copy lambda clones isolated from flow-sorted libraries and mapped to regions of chromosome 3; of these clones, 53 were found to detect useful restriction fragment length polymorphisms (RFLPs). Susan Naylor (UTHSC) described a panel of radiation hybrids constructed from a neo-marked chromosome 3 and the identification of polymerase chain reaction (PCR) primers for 40 transcribed genes on chromosome 3. Ben Carritt (MRC, London) discussed origination of the D3F15S2 locus and its related sequence on chromosome 1. Robert Gemmill (ERICR) presented a detailed pulsed-field gel map for the region p14 to p21.1; in addition, his group has identified two probes that flank the t(3;7) breakpoint. York Miller (ERICR) reported the cloning of a cDNA for aminoacylase 1 (ACY 1 at 3p21.1-3p24.2.); this widely transcribed enzyme is not detected in 20% of small-cell lung cancers.
New data on von Hippel-Lindau (VHL) syndrome linkage, reported by Bernd Seizinger (Massachusetts General Hospital), included markers developed with David Smith and Harry Drabkin; the data suggest that VHL maps in a telomeric position to RAF1, and that a single gene codes for VHL. Confirmation of the VHL-RAF1 linkage was reported by Jeff Vance (Duke University) and Eamonn Maher (Cambridge University).
Bert Zbar (NCI, FCRF) presented linkage data in VHL families and a revised linkage map of markers on 3p. Phyllis McAlpine (University of Manitoba) detailed linkage data from a family containing an inversion of chromosome 3 [inv(3)(p25q21)]. She also reported on the cloning of chromosome-3 segments in yeast artificial chromosomes (YACs).
Vincent Stanton, Hiroyuki Aburatani, and David Housman (Massachusetts Institute of Technology) reported on the conversion of several RFLPs to PCR assays, which they used in meiotic mapping studies with Norman Arnheim (University of Southern California). Takaaki Sato (Cancer Institute, Tokyo) reported a chromosome-3-specific library from which 4000 cosmid clones have been isolated. Twenty new RFLPs are being placed on the chromosome-3 linkage map.
Comparative Mapping and Informatics
Peter Lalley (Wayne State University) is using comparative mapping to investigate the evolutionarily conserved human chromosome-3-specific sequences found on mouse chromosomes 9, 6, and 16.
A database system for maintaining genetic information for chromosome 3 was discussed by Tim Bishop (Imperial Cancer Research Fund, Leeds, U.K.). The database structure is designed to incorporate the name and location of each locus and information on the sequence, polymorphism, and primer sequences required.
Chromosome-3 meeting contact:
Genetic and physical maps determined from this workshop will be included as part of the HGM 10.5 report. The next workshop on chromosome 3 will be held in about a year.
Reported by Susan Naylor
Department of Cellular and Structural Biology
University of Texas Health Science Center at San Antonio
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The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.
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