Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News Archive Edition
Human Genome News, November 1990; 2(4)
Twenty-nine scientists from eight countries gathered at St. Mary's Hospital Medical School in London September 4-5 for the First International Workshop on Human Chromosome 5, sponsored by the NIH National Center for Human Genome Research, the DOE Human Genome Program, the U.K. Medical Research Council, and GeneLabs, Inc.
Objectives of this intensive, 2-day workshop were to update the chromosome 5 consensus map and reference marker assignment, to assess resources available now or needed for future mapping efforts, and to provide input to the Chromosome 5 Committee of the Human Gene Mapping Workshop (HGM 10.5) held in Oxford September 6-11.
Genetic and Physical Mapping
Localization of spinal muscular atrophy (SMA) and familial adenomatous polyposis (FAP) genes were discussed. New linkage data placed the gene for diastrophic dysplasia (DTD)-a craniofacial dysplasia- on 5q. A large amount of new 2-point linkage data made possible the ordering of markers over much of the long arm.
Substantial progress in physical mapping was reported for chromosome 5, considering its large size. Many new markers have been placed by in situ hybridization; most are concentrated on the distal part of 5q, which seems to be rich in growth-factor and receptor genes. Detailed physical maps were presented for areas containing growth-factor genes (IL3, CSF2, IL4, and IL5) and the FAP region. An extensive collection of hybrids was reported containing translocations at 5p, which will provide a valuable resource for continued mapping in this region.
Consensus physical and genetic maps showed many informative markers, both restriction fragment length polymorphisms and microsatellites, mostly on 5q. Participants saw a clear need for more highly informative markers of the variable number of tandem repeat or microsatellite type but were pleased that most useful reference markers are freely available. Often probes that were best mapped physically had not been as well mapped genetically, and conversely. Attendees proposed that reference probes be mapped both ways by the next workshop and felt that a good start was made in providing a set of informative reference markers spanning chromosome 5.
Reported by Carol A. Westbrook
Department of Medicine
University of Chicago
and Robert T. Williamson
St. Mary's Hospital Medical School
University of London
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Human Genome Program, U.S. Department of Energy, Human Genome News (v2n4).
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