Human Genome News, November 1991; 3(4)

Human Genome Project Information. Click to return to home page.

Sponsored by the U.S. Department of Energy Human Genome Program

Human Genome News Archive Edition

HGM Group To Facilitate Work on Human YACs

At the Eleventh International Workshop on Human Gene Mapping (HGM 11) in London on August 18-22, a group of about 20 people, chaired by Charles Cantor, HUGO Vice-President (Lawrence Berkeley Laboratory) and Anthony Monaco [Imperial Cancer Research Fund (ICRF), London], met to discuss the availability and use of human yeast artificial chromosome (YAC) libraries within the genome community.

Several libraries have been distributed to a number of laboratories or are available through the human genome resource centers associated with the U.K. and European Community genome programs. These libraries include those made originally in the laboratories of Anthony Monaco, Maynard Olson (Washington University School of Medicine), Daniel Cohen (Centre d'Etude du Polymorphisme Humain, Paris), Rakesh Anand (ICI Pharmaceuticals), and Hans Lehrach (ICRF).

Discussions revealed that practices for handling YACs in the United States and Europe are quite different. In the United States, entire libraries are often available to many individual laboratories, while distribution in Europe usually has been limited to YAC arrays on filters or to pools of YACs. Laboratories screening these filters or pools then report their results to a center to receive the individual clones corresponding to the screening probe. While the U.S. system is much less restrictive because individual laboratories are free to handle the libraries and individual clones as they wish, the European approach has the advantage of accumulating a considerable amount of useful information by combining the results of many separate screening efforts.

Discussions indicated that most YAC users were not aware of the full range of libraries available and the various constraints on their use. To consider how information about YAC libraries might be spread within the community, a small group was established under the chairmanship of Gert-Jan van Ommen (Leiden University, Netherlands). Topics to be discussed by this group over the next few months:

  • compiling a periodically updated, easily accessed inventory of YAC libraries;
  • recording (in a public database) information about YAC screening with DNA probes to minimize unnecessary duplication; and
  • developing accceptable mechanisms for exchanging characterized YACs among many screening centers to promote the worldwide integration of screening efforts.

Contact:

  • Gert-Jan van Ommen
    Leiden University
    Netherlands
    Fax: (Int.) 31/71-27-60-75

Reported by Liz Evans
HUGO, London

Return to Table of Contents

The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v3n4).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.