Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, March 1993; 4(6)
GENOME CENTER:
Lawrence Livermore National Laboratory
(DOE; established 1990)
DIRECTOR:
Anthony V. Carrano
CONTACTS:
Linda Ashworth, Assistant to Center Director (510/422-5665; ashworth1@llnl.gov)
Anthony Carrano (-5698, carrano1@llnl.gov)
Lawrence Livermore National Laboratory
Human Genome Center
Biology and Biotechnology Research Program
7000 East Avenue, L-452
P.O. Box 808
Livermore, CA 94551
OTHER KEY RESEARCHERS:
Mark Batzer, Brigitte Brandriff, Elbert Branscomb, Pieter de Jong, Emilio Garcia, Richard Langlois, Greg Lennon, Harvey Mohrenweiser, Anne Olsen
MAJOR GOALS:
- Development of new cloning, mapping, instrumentation, informatics, and sequencing technologies focused on the assembly, closure, and characterization of a high-resolution ordered clone map of human chromosome 19. The final high-resolution map will consist of cosmid contigs with YACs, BACs, and PACs and an EcoR1 restriction map for the minimal spanning set of cosmids; the map will be aligned with genetic maps of chromosome 19.
- Isolation, mapping, and sequencing of chromosome 19 cDNAs with emphasis on full-length clones.
- Construction of NLGLP chromosome-specific lambda and cosmid libraries (with LANL) for distribution.
- Development of new cloning, mapping, and sequencing technologies.
MAJOR ACCOMPLISHMENTS:
- Coverage of an estimated 90% of chromosome 19 with about 800 contigs (assembled from over 12,000 cosmids, using automated fluorescence-based fingerprinting).
- Rapid closure of gaps with YACs, BACs, and PACs; 232 contigs representing about 50% of the chromosome have been regionally mapped to bands by FISH.
- Localization of more than 150 genes/polymorphic markers on the contig map. Using FISH, localization of over 500 cosmids to bands, including an ordered set of cosmids spaced an average of 1 Mb over the whole chromosome and 500 kb in selected bands.
- Organization determined for a number of gene families, including carcinoembryonic, olfactory receptor, zinc finger, cytochrome P450, and D19S11 (with several collaborators).
- Identification and characterization of the structural defect in DM, the most common form of adult muscular dystrophy (with several collaborators).
- Development of new vectors for cosmid and P1 cloning systems.
- Development of novel Alu PCR primers for fingerprinting and forensic analysis.
- Sequence determination for over 150 kb from chromosome 19 repair gene regions.
- Development of integrated mapping analysis software with interactive graphical display and linkage to local and national databases.
- Development of a high-speed flow cytometer and sorter for cell and chromosome purification.
- Construction of NLGLP chromosome-specific lambda and cosmid libraries from sorted chromosomes 3, 7, 9, 12, 18, 19, 21, 22, X, and Y.
AVAILABLE RESOURCES:
- NLGLP large-insert lambda and cosmid chromosome-specific libraries.
- Cosmid filters, FISH mapping, and cDNA and YAC/BAC/PAC screening for chromosome 19 probes.
- Assistance in database development, systems management, networking, and in contig assembly by fingerprinting and automated restriction mapping.
- Graduate and postdoctoral research training through the Institute of Genetics and Genomics at LLNL (Mohrenweiser, 510/423-0534).
Genome Centers Acronym List
HGMIS Staff
Return to List of Genome Centers
Return to Table of Contents
The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v4n6).