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Human Genome News Archive Edition

Human Genome News, March 1993; 4(6)

GENOME CENTER:

Lawrence Livermore National Laboratory

(DOE; established 1990)

DIRECTOR:
Anthony V. Carrano

CONTACTS:
Linda Ashworth, Assistant to Center Director (510/422-5665; ashworth1@llnl.gov)
Anthony Carrano (-5698, carrano1@llnl.gov)
Lawrence Livermore National Laboratory
Human Genome Center
Biology and Biotechnology Research Program
7000 East Avenue, L-452
P.O. Box 808
Livermore, CA 94551

OTHER KEY RESEARCHERS:
Mark Batzer, Brigitte Brandriff, Elbert Branscomb, Pieter de Jong, Emilio Garcia, Richard Langlois, Greg Lennon, Harvey Mohrenweiser, Anne Olsen

MAJOR GOALS:

  • Development of new cloning, mapping, instrumentation, informatics, and sequencing technologies focused on the assembly, closure, and characterization of a high-resolution ordered clone map of human chromosome 19. The final high-resolution map will consist of cosmid contigs with YACs, BACs, and PACs and an EcoR1 restriction map for the minimal spanning set of cosmids; the map will be aligned with genetic maps of chromosome 19.
  • Isolation, mapping, and sequencing of chromosome 19 cDNAs with emphasis on full-length clones.
  • Construction of NLGLP chromosome-specific lambda and cosmid libraries (with LANL) for distribution.
  • Development of new cloning, mapping, and sequencing technologies.

MAJOR ACCOMPLISHMENTS:

  • Coverage of an estimated 90% of chromosome 19 with about 800 contigs (assembled from over 12,000 cosmids, using automated fluorescence-based fingerprinting).
  • Rapid closure of gaps with YACs, BACs, and PACs; 232 contigs representing about 50% of the chromosome have been regionally mapped to bands by FISH.
  • Localization of more than 150 genes/polymorphic markers on the contig map. Using FISH, localization of over 500 cosmids to bands, including an ordered set of cosmids spaced an average of 1 Mb over the whole chromosome and 500 kb in selected bands.
  • Organization determined for a number of gene families, including carcinoembryonic, olfactory receptor, zinc finger, cytochrome P450, and D19S11 (with several collaborators).
  • Identification and characterization of the structural defect in DM, the most common form of adult muscular dystrophy (with several collaborators).
  • Development of new vectors for cosmid and P1 cloning systems.
  • Development of novel Alu PCR primers for fingerprinting and forensic analysis.
  • Sequence determination for over 150 kb from chromosome 19 repair gene regions.
  • Development of integrated mapping analysis software with interactive graphical display and linkage to local and national databases.
  • Development of a high-speed flow cytometer and sorter for cell and chromosome purification.
  • Construction of NLGLP chromosome-specific lambda and cosmid libraries from sorted chromosomes 3, 7, 9, 12, 18, 19, 21, 22, X, and Y.

AVAILABLE RESOURCES:

  • NLGLP large-insert lambda and cosmid chromosome-specific libraries.
  • Cosmid filters, FISH mapping, and cDNA and YAC/BAC/PAC screening for chromosome 19 probes.
  • Assistance in database development, systems management, networking, and in contig assembly by fingerprinting and automated restriction mapping.
  • Graduate and postdoctoral research training through the Institute of Genetics and Genomics at LLNL (Mohrenweiser, 510/423-0534).

Genome Centers Acronym List


HGMIS Staff

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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v4n6).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.