Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News Archive Edition
Human Genome News, April-June 1996; 7(6)
Keith Elliston (Merck & Co.) reported on the analysis of sequence data produced in the Washington University (WU) - Merck EST sequencing project. Sequencing was done on cDNA clones from Bento Soares' high-quality normalized libraries that were arrayed and distributed by the IMAGE consortium [HGN 6(6), 3 and 7(5), 1-2, 7].The project's goal is to develop a nonredundant resource, the Merck Gene Index, composed of one cDNA clone per expressed human gene. More than 245,000 EST sequences from this project have been submitted to public databases by WU, representing over 91 Mb of DNA from about 150,000 clones.
Elliston described in some detail the work begun at Merck to develop the set of nonredundant sequences. The latest results on creating the preliminary index are available via WWW [IMAGE http://www.imageconsortium.org/]. EST coverage of known transcripts is high (65% of known genes from GenBank), 5' end coverage of genes hit is relatively high (about 30%), and 3' ESTs extend known transcripts (27%). The rate of chimerism is extremely low. Commenting on the initial data-analysis results, Elliston characterized the project as informative, high quality, and worthwhile.
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Human Genome Program, U.S. Department of Energy, Human Genome News (v7n6).
The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.
Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.