Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News Archive Edition
Human Genome News, April-June 1996; 7(6)
Santa Fe '96
Julie Korenberg (Cedars-Sinai Research Institute) described the practical value of having a single resource of mapped clones for studying human genetics at the sequence, gene, chromosome, and whole-genome levels. She reported on progress toward creating such a resource and its early applications for mapping, sequencing, gene isolation, molecular cytogenetics, and genome-organization analysis.
Ultimately, the Mapped BAC-PAC Resource will represent 0.8 to 1.2x of the human genome (about 18,000 to 20,000 clones, covering over 70%) in a stable framework resource. The Resource will be based on BACs and PACs from Melvin Simon and Pieter de Jong and integrated at 1000 to 5000 loci with the radiation hybrid, genetic, and STS maps. Korenberg chose BACs and PACs because they are relatively stable, easy to use, and useful for sequencing and probes.
By using FISH, almost 4000 BACs and PACs (3638 and 242, respectively) have been assigned to regions of 2 to 6 Mb (average insert sizes, 125 to 150 kb). These BACs and PACs represent 17 to 20% of the human genome. The rate of chimerism is very low (8% for BACs and 2.5% for PACs).
BACs have been obtained for all 24 chromosomes and 17 of the centromeres. Specific BACs for 11 telomeres and nonspecific BACs for 19 telomeres are available, and 100 BACs have been mapped to single bands in the mouse genome.
Tom Hudson (Whitehead-MIT) is screening 5000 markers against the subset of nearly 18,000 BACs; so far, agreement with MIT chromosome and region assignment has been 80% for 216 markers. All common markers are also mapped on STS and radiation hybrid maps.
The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v7n6).
The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.
Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.