HGPI

Human Genome Project Information Archive
1990–2003

Archive Site Provided for Historical Purposes


Sponsored by the U.S. Department of Energy Human Genome Program

Human Genome News Archive Edition
go to list of issues »

Human Genome News, January 1998; 9:(1-2)

Table 1.  Large DNA Cloning Systems

  YAC BAC PAC HAEC MAEC
Host Yeast Bacteria Bacteria Human Mouse
Genetic Elements ARS/CEN F episome P1 bacteriophage OriP/EBNA-1 HAEC OriP/EBNA-1
DNA Status Linear Circular Circular Circular Circular
Insert size (kb) 100 to 2000 40 to 300 40 to 250 80 to 350 80 to 120
Copy#/cell 1 1 1 ~1 to 10 ~1 to 10
Amplification - - 20x 1000x (+)
Viral Stock - - + + (ND*)
Non-Recombinant - + + - (ND)
Chimeras + - - - (ND)
Stability (+ -) + + + (+)
Major application STS linkage, contig maps Contig maps, sequencing Contig maps, sequencing Functional analysis Functional analysis
References Burke et al. (1987) Shizuya et al. (1992) loannou et al. (1994) Sun et al. (1994) Kelleher et al. (1997)

 * not determined.

Back to "Large-Insert Cloning Vectors Aid Function Studies"



Back to Table of Contents

The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v9n1).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.