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Excerpted from the report:


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PRIORITY SETTING MEETING FOR MOUSE GENOMICS AND GENETICS RESOURCES:

FIRST FOLLOW-UP MEETING

OCTOBER 5, 1998

SUMMARY OF MEETING: POINTS OF CONSENSUS AND ACTION ITEMS

A. STRUCTURAL GENOMICS
1. The Mouse Strain to be Sequenced
2. Sequencing Strategy

The recommended strategy for sequencing the mouse is to build the physical map as the sequence is generated. A three-phase strategy was proposed:

Phase I (Random): Researchers would sequence individual BAC clones at random until 30 percent of a 15X library was sequenced. The BAC clones would be selected at random or based on scientific interest. BACs would be sequenced and STSs from sequenced BACs would then be mapped onto a common high resolution RH mapping panel. All information would be entered into a common database.

Phase II (Directed): The sequencing strategy would change over to extending sequencing off the ends of BACs using information from the BAC fingerprinting or the BAC end sequencing projects to direct the extension. This phase would be in place until about 90 % of the genome had been sequenced.

Phase III (Closure): This might require special libraries (library made with different enzymes, smaller insert size libraries, etc) for gap filling.

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The complete text of this report will be available after review by the participants. For details contact Betty Graham at NIH/NHGRI


Last modified: Wednesday, October 22, 2003

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