Sequencing and Analysis of Full Length cDNAs in the German cDNA Network

Stefan Wiemann
German Cancer Research Center
Dept. Molecular Genome Analysis
Im Neuenheimer Feld 506
69120 Heidelberg, Germany
telephone: +49 6221 42 4702
fax: +49 6221 42 4704
email: s.wiemann@dkfz.de
prestype: Platform
presenter: Stefan Wiemann

Stefan Wiemann1, Bernd Weil, Ruth Wellenreuther, Sabine Krieger, Wilhelm Ansorge, Michael Böcher, Helmut Blöcker, Helmut Blum, Andreas Düsterhöft, Jürgen Lauber, Andreas Beyer, Karl Köhrer, Christian Gruber, Hans-Werner Mewes, Brigitte Obermaier, Birgit Ottenwälder, Dagmar Heubner, Rolf Wambutt, Jeremy Simpson, Rainer Pepperkok, Annemarie Poustka
1Molecular Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld 506, D-69120 Heidelberg Germany, and the German cDNA Network

We have formed a network in the frame of the German Genome Project aiming at the generation and sequencing of novel full-length cDNAs, and the comprehensive functional analysis the deduced proteins. The project started in September 1997. Over 3,500 cDNAs (> 8.6 Mb) have been sequenced since.

We use the set of fully sequenced clones in combination with the EST-sequenced clones to generate a minimal set of full-length clones for employment in subsequent functional analysis. All sequences are first analyzed for possible protein function in silico. To systematically characterize function of the encoded proteins in vivo, we initially determine the subcellular localization of the proteins. A progress report of the network activities and the achievements will be presented.

In future projects cellular assays will be applied to comprehensively unravel the role of most or all proteins in view of their cellular function(s), the pathways they are involved in and possible disease relations.



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