Beyond the Identification of Transcribed Sequences:
Functional, Evolutionary and Expression Analysis
12th International Workshop
October 25-28, 2002
Washington, DC


List of Abstracts * Speakers * Organizers * Authors * Original Announcement


Mapping the Transcriptome

Thomas Freeman
HGMP-Resource Centre, Hinxton, CB10 1SB UNITED KINGDOM
Telephone: 44 1223 494552
Fax: 44 1223 494512
Email: tfreeman@hgmp.mrc.ac.uk

Complete sequencing of the human, and more recently the mouse genome, provides an invaluable resource in our efforts to understand many aspects of biological function in both health and disease. Expression profiling, in particular using high-density arrays of gene-specific DNA probes, has firmly been demonstrated to be one of the most powerful and direct ways of translating sequence data into functional information. However, in spite of the fact that this technology has been in use for a number of years, we still have very little appreciation of when, where and to what degree most genes are expressed, at either the tissue or cellular level. This information is vital for our ability to appreciate transcriptome diversity, and the regulation and function of many genes. For a number of years now we have been endeavouring to map systematically in high throughput assays the expression of genes over a wide range of normal tissues and cell types with the aim of stabling databases of gene expression profiles. Initially, our efforts to map the mammalian transcriptome employed techniques such as RT-PCR but more recently we have used microarrays. I will discuss our findings and our continuing efforts to establish an "atlas" of gene expression. I will also discuss other aspects of expression analysis technology, and its use and development at the HGMP-RC.



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List of Abstracts * Speakers * Organizers * Authors * Original Announcement


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