Beyond the Identification of Transcribed Sequences:
Functional, Evolutionary and Expression Analysis
12th International Workshop
October 25-28, 2002
Washington, DC


List of Abstracts * Speakers * Organizers * Authors * Original Announcement


Kazusa cDNA Project 2002: Of Mice and Men

Osamu Ohara
Kauzsa DNA Research Insitute/RIKEN Research Center for Allergy and Immunology,
Kisarazu, 292-0818 JAPAN
Telephone: +81-438-52-3913
Fax: +81-438-52-3914
Email: ohara@kazusa.or.jp

We have conducted a human cDNA sequencing project for identification of unknown human transcripts in the past 8 years. A distinctive point of our project from other cDNA sequencing projects is in that we have focused our sequencing efforts on long cDNA clones (>4 kb), particularly those encoding large proteins in brain. This approach has brought us a unique cDNA resource consisting of large cDNA clones for newly identified human genes, which are known as KIAA cDNAs. The number of KIAA cDNAs has exceeded 2000 this year (the total number of the sequenced nucleotide residues, about 10 Mb) and we have made them publicly available. Because the number of genes encoding large proteins (>100 kDa) is always smaller than 10% of the total number of genes in eukaryotes, the number of human genes encoding large proteins is likely less than 3000. If this estimate is correct, we must have certainly entered endgame of the identification of human transcribed sequences expressed in the brain. Thus, this has strongly urged us to move toward the second phase of our project, i.e., toward functional analyses of the newly identified transcripts and their products through exploiting fully the accumulated cDNA resource. In this regard, we have already started to perform expression profiling of KIAA genes on nylon cDNA microarray and to analyze protein networks involving KIAA proteins on the basis of yeast two-hybrid screening.

One of our goals beyond the identification of KIAA transcripts is to understand the function of KIAA proteins in vivo. For this purpose, we consider it reasonable to perform analysis of KIAA proteins in mice rather than in human because isolation of human proteins from various tissues might raise a serious ethical issue and various types of genetically engineered mice are currently available. We have thus initiated to prepare a set of mouse KIAA cDNAs for analysis of their products in vivo. Besides the characterization of KIAA proteins in vivo, the isolation of mouse KIAA cDNAs has enabled us to experimentally evaluate the integrity of KIAA cDNA clones through comparison of human and mouse KIAA cDNAs. Furthermore, in this mouse KIAA cDNA project, we have also planned to prepare a set of antibodies against all the mouse KIAA proteins, which will provide us with a powerful tool to explore the structure and function of the mouse KIAA proteins in vivo. In this presentation, more details regarding the current status of human and mouse KIAA cDNA projects will be described.



  Abstract List


List of Abstracts * Speakers * Organizers * Authors * Original Announcement


Genetic Meetings