Beyond the Identification of Transcribed Sequences:
Functional, Evolutionary and Expression Analysis
12th International Workshop
October 25-28, 2002
Washington, DC

List of Abstracts * Speakers * Organizers * Authors * Original Announcement

Two Pancreas Clone Sets and PancChips, New Resources for Diabetes

Marie Scearce and Klaus Kaestner
University of Pennsylvania, Philadelphia, PA 19104 USA
Telephone: 215-743-6372
Fax: 215-573-5892

In order to investigate gene expression in the developing pancreas, we have compiled two pancreas-enriched clone-sets and corresponding microarrays. Our initial 3.4K pancreas clone set contains unique clones representing genes expressed in the pancreas as well as known genes of interest which were identified through preliminary array experiments with commercially available arrays and through a search of dbEST libraries for libraries derived from pancreatic tissue. Non-redundant clones (3139) from all sets were obtained from the IMAGE consortium these clones were re-sequenced by the Genome Sequencing Center and 1,898 of the clones were sequence verified. In addition to this clone set we have identified approximately 260 clones of interest from know signal transduction pathways and other genes of interest and added these to the clone set. Our second clone set is compiled from libraries prepared in the Melton and Kaestner laboratories. A unique set of 7227 clones from these libraries were identified, of which approximately 60% are either novel clones or without functional groups previously deposited in dbEST. These clones were re-sequenced by the IMAGE consortium. The combined clone set consists of approximately 11,000 cDNAs and ESTs representing over 10,000 non-redundant mRNAs expressed in mouse pancreas. Plasmid DNA from the clones was isolated and the inserts were amplified with PCR. The purified PCR products were printed on poly-L-lysine coated glass microscope slides. We have used PancChip 2, (from the 3.4K clone set) to study the expression of genes in the developing pancreas at pc14.5, pc16.5, pc18.5, newborn, 7-day-old and adult mice.

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