helix

Exceptional Chromosome Regions Workshop I

Home

 

Abstract

Evan E. Eichler
Case Western Reserve University

A series of chromosome 19, p12-specific repeat probes have been generated and have been used to screen two genomic library sources (RPCI11 and CIT-D BAC libraries). Probes were generated using a degenerate 19p12-specific beta-satellite PCR assay. The initial target region focussed primarily on the existing 8 gap regions within the physical map of 19p12. To date, a total of 403 BAC clones have been identified (192 RPCI-11 BACs and 211 CIT-D BACs) representing an estimated ~2.5 Mb of the 19p12 region. A subset (74) of these BAC clones have been selected for BAC-end sequence analysis. These BAC-ends in conjunction with existing BAC-end sequences deposited in GenBank have successfully been used to extend 12 of the 16 BAC contigs in the region. This includes extension of the most proximal contig to include the transition into heterochromatic alpha-satellite DNA. An additional 120 kb of pericentromeric DNA has been added to the map, including higher-order alpha satellite DNA. Four of the original eight gap regions have been tentatively closed based on this analysis. In collaboration with Dr. Anne Olsen (LLNL), 57 BACs have been selected for integration into the 19p12 physical map. Incorporation of these clones into the 19p12 physical map is currently being used to complete sequence analysis in this region.

The short arm of chromosome 16 is also under analysis.


Last modified:

Base URL: www.ornl.gov/meetings/ecr1/

Site sponsored by the U.S. Department of Energy Office of Science, Office of Biological and Environmental Research, Human Genome Program