TRANSCRIPTOME 2002: From Functional Genomics to Systems Biology
March 10-13, 2002
Seattle, Washington, USA

Gene catalog of mouse stem cells and early embryos

Minoru S. H. Ko1, Yulan Piao1, Carole A. Stagg1, Patrick Martin1, Dawood B. Dudekula1, Yong Qian1, Lakshmi Kosuru1, Vincent VanBuren1, Tetsuya S. Tanaka1, Saied A. Jaradat1, Mark G. Carter1, Wendy L. Kimber1, Kazuhiro Aiba1, Toshio Hamatani1, Toshiyuki Yoshikawa1, Janet Kelso2, Winston Hide2.

1Developmental Genomics and Aging Section, Laboratory of Genetics, National Institute on Aging (NIA), National Institutes of Health (NIH), Baltimore, MD 21224, USA

2South African National Bioinformatics Institute, University of the Western Cape, Bellville, South Africa

We report here our continued efforts to assemble a mouse gene catalog from early embryonic stages as well as embryonic and adult stem cells. To overcome the scarcity of materials in these stages, we recently developed a method to construct long-insert enriched cDNA libraries from submicrogram amounts of total RNA. This new method allowed us to add ~80,000 new ESTs, which may represent full-length cDNAs, to our previous collection of ~80,000 ESTs. These ESTs were clustered into ~26,000 unique gene collections, which added ~11,000 new cDNA clones to the NIA Mouse 15K cDNA clone set. The new 11K clone set is now being single-colony isolated and sequence-verified. These ESTs and cDNA clone collections will provide essential tools to study the mammalian development as well as the fundamentals of stem cells, and provide initial hints about the differential nature of embryonic and adult stem cells.


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