TRANSCRIPTOME 2002: From Functional Genomics to Systems Biology
March 10-13, 2002
Seattle, Washington, USA

Kazusa cDNA Project 2002: From Transcript to Protein

Osamu Ohara 1,2, Reiko Kikuno1, and Takahiro Nagase1, 1Kazusa DNA Research Institute, Kisarazu, JAPAN, 2RIKEN Research Center for Allergy and Immunology, Kisarazu, JAPAN

We have conducted a human cDNA sequencing project for the identification of unknown human transcripts in the past seven years. A distinctive characteristic that separates our project from other cDNA sequencing projects is that we have focused our sequencing efforts on long cDNA clones (>4 kb), particularly those encoding large proteins (>1000 amino acid residues). This approach has resulted in a unique cDNA resource consisting of nearly 2000 large cDNA clones for newly identified human genes, which are known as KIAA genes. The detailed information regarding KIAA genes can be accessible through HUGE protein database at http://www.kazusa.or.jp/huge. As we enter the end game of identification of human transcripts coding for unknown large proteins, we are gradually shifting our aim toward characterization of proteins encoded by KIAA genes. Besides functional characterization of KIAA gene products (e.g., systematic search for binding partners of KIAA gene products in vitro), our important aim is to link the KIAA transcripts with their protein products in vivo. To achieve this, we are planning to extensively use antibodies as specific detection reagents for the KIAA gene products. The set of expression-ready KIAA cDNA clones and antibodies against the KIAA gene products will provide us with a powerful tool for filling the gap between the mammalian transcriptome and proteome.


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