TRANSCRIPTOME 2002: From Functional Genomics to Systems Biology
March 10-13, 2002
Seattle, Washington, USA


Production and Use of DNA Microarrays for Studies of Aging

Mark W. Eshoo and Krysta Felkey, Buck Institute for Age Research, Novato, CA

The Buck Institute for Age Research is a non-profit, independent, research institute located 20 miles north of San Francisco in Marin County California.  Research at the Buck Institute is focused upon aging and diseases of aging such as Cancer, Parkinson's and Alzheimer's disease. To support researchers in the field of Aging the Buck Institute has set up a high throughput Genomics Core facility that offers researcher investigators both here at the Buck Institute and at other institutions an integrated gene expression resource center.  The Genomics Core performs all the steps necessary for microarray analysis of gene expression.  The Genomics Core also assists the researchers in the experimental design of their gene expression studies and provides all the resources necessary for the performance and of microarray gene expression studies from samples as small as a few hundred cells.

One of the key strengths of the Buck Institute's Genomics Core is our capacity to efficiently produce the tens of thousands of DNA samples required to produce our DNA microarrays using the 384 well microtiter plate.  This high throughput format combined with our ability to robotically select individual genes from our collection of 85,000, unique and sequence verified cDNA clones enables the Genomics Core to quickly produce microarrays that can be custom designed to meet the individual researcher's needs.

The Genomics Core has developed a number of human, mouse, Drosophila and C. elegans DNA microarrays that are specifically designed for studies of aging and age related diseases. Data will be presented on microarray methods development and examples of data generated by the Genomics Core from studies of aging.

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