TRANSCRIPTOME 2002: From Functional Genomics to Systems
Expression of Cytokines and Growth Factors in Aging and hTERT-transfected Human Fibroblasts
Yukari Kanzaki, Toshinori Ide, Hiroshima University School of Medicine, Hiroshima, JAPAN
Telomerase is the unique reverse transcriptase that synthesizes telomeric DNA onto chromosomal ends using its RNA component as a template. Telomerase expressed in the most cancer cells, but not somatic cells except germline cells and stem cells. We surveyed, using membrane array hybridization, the expression of some growth factors and cytokines in human fibroblasts transfected with human telomerase reverse transcriptase subunit (hTERT) cDNA. Twelve genes out of 268 were induced and 7 out of 268 were repressed in hTERT-transfected fibroblasts. Telomerase are specialized structure at the end of eukaryotic chromosomes and concerned with chromosomal stability. Telomere DNA shortens by 50-200 b.p. per cell division and telomere length has been proposed as a counting mechanism of cell division number that controls cellular senescence. We have examined the changes in gene expression in accordance with aging in vitro. Moreover we examined whether telomere elongation rescues expressional changes, which occur during aging.
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