TRANSCRIPTOME 2002: From Functional Genomics to Systems
Quality Control of Microarray Samples Using Lab-on-a-Chip Technology
Odilo Müller1, Monika Dittmann1, Samar Lightfoot2, Kathi Williams3, and Robert Dubrow3, 1Agilent Technologies, Waldbronn, GERMANY, 2Agilent Technologies, Palo Alto, CA, 3Caliper Technologies Corp. Mountain View, CA
Microarray experiments allow the fast and efficient assessment of the expression of thousands of genes in one single experiment. The quality of the array data is largely dependent on the quality of the array itself, as well as on the quality of the labeled probes that are hybridized to the array. The Agilent 2100 bioanalyzer – the first commercially available Lab-on-a-Chip system – is the tool of choice for the quality assessment of RNA samples at different steps within the microarray workflow. A set of new RNA assays has been developed to better suit the needs of microarray users. Sample alignment using an internal marker allows better comparison of different samples, also providing rough size estimation. Quantitative measurement of RNA samples allows in many cases to forgo UV measurements. For the analysis of RNA samples from micro-dissected tissue, a new assay is being developed, which allows the detection of RNA down to 100 pg of sample.
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