TRANSCRIPTOME 2002: From Functional Genomics to Systems
Gene Expression Profiling on Radiation Responses
Woong-Yang Park, Chang-Il Hwang, Chang-Nim Im, Min-Ji Kang, Jang-Hee Woo, Ju-Hoon Kim, Kyoung-A Kim1, Hyoung-Jin Yoo1, Yun-Sil Lee2, Su-jae Lee2, Jeong-Sun Seo, Department of Biochemistry and Molecular Biology and Ilchun Molecular Medicine Institute, Seoul National University College of Medicine, 1Macrogen Inc., 2Korea Cancer Center Hospital, Seoul, KOREA
Frequent mutations in tumor cells prevent the expression of p53, which is the key molecule to determine the responses to ionizing radiation. Gene expression profiling in p53-deficient cells will unveil the p53-independent mechanisms of checkpoint control, repair and cell death following gamma-irradiation. Jurkat T cells with nonsense mutation on p53 and PTEN were exposed to 4Gy to 16Gy gamma-irradiation. Using 2,400 human cDNAs, the gamma-irradiation-responsive genes were examined by microarray. We could find the induction of phospholipases after the gamma-irradiation. Other radical scavenger was also induced, which might explain the interpersonal difference in radio-resistance. However, we could not find any significant changes in cell cycle-related genes. Using 384 genes related to gamma-irradiation, different tumor cell lines and peripheral blood mononuclear cell (PBMC) were found to be similar in expression pattern. From these results, we could list the novel radiation-related genes and also suggest the possibility of usage of these biomarkers in DNA chip for checking radiation exposure.
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