TRANSCRIPTOME 2002: From Functional Genomics to Systems
Genetic Hardware and Genetic Software after Human Genome Project
Shuwei Yang, GeneCopoeia, Inc., Frederick, MD
With the progress of Human Genome Project, other animal genomes and pathogenic microorganism genome projects, many full-length genes were discovered and more and more will be added into the list. I proposed the two sections of the post-genome project, Genetic Software and Genetic Hardware. The opportunities and challenges of these two sections will be discussed. The contents of the Genetic Hardware Section are high speed computer, high accuracy genetic data, high accuracy of full-length cDNA clones, high efficiency gene transfer vector and host, and high specific and sensitive molecular monitoring system in vitro and in vivo. The study of the interaction among proteins encoding by human and pathogenic microorganism genes is the next wave of the life science. The ORFs of these genes have to be transferred from one vector to another very frequently in order to produce the gene product (protein) in vitro and in vivo. Classic subcloning methods can not meet the requirement of rapidly growing of the studying of function of many discovered genes. New technologies of semi-automation we have invented to accelerate re-cloning of the ORFs of full-length genes with higher fidelity into suitable vector(s) for specific host(s) and the QC/AC will be discussed. The problems such as the difference in the sequence analysis of the ORFs from public cDNA database and genomic annotation will be explored and discussed.
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