TRANSCRIPTOME 2002: From Functional Genomics to Systems
FLJ cDNA Project in Japan
Sumio Sugano, Human Genome Center, The Institute of Medical Science, The University of Tokyo, Tokyo, JAPAN
Although a huge data of draft and finished sequences of the human genome are now available, it is still not a trivial task to identify genes from genome sequences. Thus, a full-length complementary DNA (cDNA), which is a complete DNA copy of mRNA, is required for the identification of a gene and the determination of its structure. We are now participating FLJ cDNA project to collect and sequence full-length cDNA clones under support of ministry of economy, industry and international trade. In this project, we extensively used cDNA libraries made by Oligo-capping method, a cap targeted selection procedure for full-length cDNAs. So far, we have accumulated more than 800,000 5' end sequences through random sequencing of cDNA clones from about 100 kinds of human cDNA libraries. Among them, 12340 clones have been fully sequenced. The accuracy of the sequences was more than 99.95%. The average length of cDNAs whose sequence were determined was about 2200bp, which distributed from 1kb to 5kb. Furthermore we could cluster 5' end of many mRNAs (more than 5000 genes), which could be mapped to onto the human draft genomic sequence. The detail of this project will be presented.
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