Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):4
Genome Therapeutics Corporation
Genome Sequencing Center
GENOME THERAPEUTICS CORPORATION (COLLABORATIVE RESEARCH DIVISION) GENOME SEQUENCING CENTER
- (NIH, established 1994)
- JEN-I MAO, Director
- CONTACT: Mao (617/893-5007 x242, Fax: /642-0310, mao@cric.com); 100 Beaver St.; Waltham, MA 02154.
OTHER KEY RESEARCHERS
- George Church (Harvard Medical School)
- Ronald Lundstrom
- Peter Richterich
- Hershel Safer
- Douglas R. Smith
MAJOR GOALS
- Improvement of the overall throughput and cost-efficiency of multiplex sequencing by 10 times.
- Sequencing of Mycobacterium leprae and Mycobacterium tuberculosis genomes and large regions of the human genome.
- Automation and improvement of front-end multiplex sequencing protocols.
- Development of a high-throughput integrated system for automated hybridization and infrared fluorescence detection.
- Development and implementation of software tools to support sequence-production needs including base calling, sequence assembly, contig editing, and sequence analysis.
MAJOR ACCOMPLISHMENTS
- Completion of 1-Mb mycobacterial and human genomic sequences using software REPLICA and GTAC developed by Church, Leon Mintz, and Gary Gryn (Howard Hughes Medical Institute, Harvard Medical School). About 900 kb are in GenBank.
- Development of improved internal sequencing standard and a set of 20-plex phagemid vectors for chemical and dideoxy multiplex sequencing.
- Demonstration of highly sensitive infrared detection from membranes of multiplex sequencing.
- Construction of prototype for automated hybridization of multiplex membranes.
- Development of software modules and programs for automated primer picking and assembly verification.
- Development of improved software for lane definition and band location on multiplex internal standards and near completion of improved base-calling software.
- Development of automated management tools for maintenance of individual sequencing projects.
- Development of program to assemble cosmids containing long repeated regions. When available, the mate information from double-ended sequencing is incorporated.
AVAILABLE RESOURCES
- Mycobacterial and human sequence data.
- Improved multiplex phagemid vectors.
- Test data set for sequence assembly.
- X-gel, multiplex sequencing software for semi-automated lane finding and image analysis.
- Human chromosome 10 physical mapping data and mapped cosmid and yeast artificial chromosome clones.
HGMIS staff
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).
