Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):4
Lawrence Livermore National Laboratory
LAWRENCE LIVERMORE NATIONAL LABORATORY (LLNL)
- (DOE, established 1990)
- ANTHONY V. CARRANO, Director
- CONTACTS: Linda Ashworth, Assistant to Center Director (510/422-5665, Fax: -2282, ashworth1@llnl.gov) or Carrano (-5698, carrano1@llnl.gov); LLNL; Human Genome Center; 7000 East Ave., L-452; P.O. Box 808; Livermore, CA 94551.
OTHER KEY RESEARCHERS
- Joe Balch
- Mark Batzer
- Brigitte Brandriff
- Elbert Branscomb
- Emilio Garcia
- Jeff Garnes
- Jeff Gingrich
- Jane Lamerdin
- Richard Langlois
- Greg Lennon
- Ray Mariella
- Harvey Mohrenweiser
- Anne Olsen
- Tom Slezak
MAJOR GOALS
- Development of new cloning, mapping, and sequencing technologies focused on the assembly, closure, and characterization of a high-resolution ordered clone map of human chromosome 19. The final high-resolution map will consist of cosmid contigs with yeast artificial chromosomes, bacterial artificial chromosomes, and P1 artificial chromosomes and an EcoR1 restriction map for the minimal spanning set of cosmids; the map will be aligned with genetic maps of chromosome 19.
- Isolation, mapping, and sequencing of chromosome 19 cDNAs with emphasis on full-length clones.
- Physical mapping and sequencing of targeted gene families in human and other relevant species.
- Comparative sequencing of selected mouse and human genomic regions.
- Development of software solutions for data management and integrated map display for physical mapping.
- Construction of National Laboratory Gene Library Project chromosome-specific lambda and cosmid libraries (with LANL) for distribution.
- Development of instrumentation in support of physical mapping and DNA sequencing.
- Transfer of technology and knowledge to industry, universities, and the public.
MAJOR ACCOMPLISHMENTS
- Coverage of an estimated 95% of chromosome 19 in cosmid contigs. Over 85% of the chromosome is spanned by ordered islands of cosmid, yeast artificial chromosome, bacterial artificial chromosome, P1 artificial chromosome, and P1 phage clones, with distances determined between >200 selected cosmids spaced an average 260 kb apart.
- Fluorescence in situ hybridization localization of over 400 cosmids to chromosome bands, including an ordered set of 190 cosmids spaced an average 260 kb apart.
- Localization of over 400 genes, genetic markers, and other loci at the cosmid level on the chromosome 19 map.
- Fine-scale mapping in selected regions, including the cytochrome P450, pregnancy-specific glycoprotein, fucosyltransferase, and zinc finger genes or gene families.
- Construction of EcoR1 complete digest restriction maps spanning 32 Mb of chromosome 19.
- Establishment of Mosaic access to current chromosome 19 physical map.
- Identification of novel Alu repeat sequences for studying human genetic variation.
- Sequence determination and comparison of over 250 kb from chromosome 19 regions, including DNA repair genes and their rodent homologs.
- Development of instrumentation supporting high-density filter production, DNA sequencing, and flow cytometry.
- Construction of National Laboratory Gene Library Project chromosome-specific cosmid libraries from sorted chromosomes for chromosomes 1, 2, 3, 7, 9, 12, 18, 19, 21, 22, X, and Y.
- Development of integrated mapping analysis software with interactive graphical display and linkage to local and national databases.
AVAILABLE RESOURCES
<<ul> National Laboratory Gene Library Project large-insert lambda and cosmid chromosome-specific libraries. Cosmid filters, fluorescence in situ hybridization mapping, and cDNA and yeast artificial chromosome, bacterial artificial chromosome, P1 artificial chromosome screening for chromosome 19 probes. Assistance in database development, systems management, networking, and contig assembly by fingerprinting and automated restriction mapping. Graduate and postdoctoral research training through the Institute of Genetics and Genomics at LLNL (Mohrenweiser, 510/423-0534). URL: http://bbrp.llnl.gov/bbrp/genome/genome.html
HGMIS staff
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).
