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Human Genome News Archive Edition

Human Genome News, Nov. 1994; 6(4):8

University of California, Irvine

(NIH, established 1993)
John D. McPherson, Co-Director
CONTACTS: Wasmuth (714/856-7067), McPherson (-8242), or Judy Brown, Administrative Assistant (-7067); (Center Fax: /725-3403); UCI; Natl. Hum. Gen. Res. Center; Coll. of Med.; Irvine, CA 92717.


  • Construction of 150-kb-resolution radiation hybrid map of human chromosome 5 including all highly polymorphic short tandem repeat polymorphism markers and every known gene on the chromosome.
  • Establishment of a radiation hybrid contig (from single 5- to 7-Mb fragments of chromosome 5) that spans the entire chromosome in minimally overlapping segments.
  • Isolation of about 100 cosmids from each of 30 bins defined by the final radiation hybrid contig.
  • Isolation and sequencing of about 30 exons from each of the same 30 bins via exon amplification of pooled cosmids.


  • Placement of 1200 loci on chromosome 5 radiation hybrid map. Order of 850 loci was established with odds >10,000:1.
  • Identification of 7 single-fragment radiation hybrids with different, defined segments of 5q; total coverage of 5q is about 30%.
  • Establishment of complete cosmid contig spanning about 3 Mb in 5q31-32.
  • Isolation of the hyperekplexia and achondroplasia genes.


  • Natural deletion hybrids of chromosome 5.
  • Selected radiation hybrids with defined 5 to 10 Mb of 5q. (Entire set will become available when the complete RH "contig" is complete.).
  • Set of about 300 cosmids for fluorescence in situ hybridization, distributed relatively evenly along the chromosome.
  • Placement of markers (short tandem repeat polymorphisms or genes) from other laboratories on the RH map with data being maintained confidentially for 3 months..

HGMIS staff

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Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

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