Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):8
University of California, Irvine
UNIVERSITY OF CALIFORNIA, IRVINE (UCI)
- (NIH, established 1993)
- JOHN J. WASMUTH, Director
- John D. McPherson, Co-Director
- CONTACTS: Wasmuth (714/856-7067), McPherson (-8242), or Judy Brown, Administrative Assistant (-7067); (Center Fax: /725-3403); UCI; Natl. Hum. Gen. Res. Center; Coll. of Med.; Irvine, CA 92717.
MAJOR GOALS
- Construction of 150-kb-resolution radiation hybrid map of human chromosome 5 including all highly polymorphic short tandem repeat polymorphism markers and every known gene on the chromosome.
- Establishment of a radiation hybrid contig (from single 5- to 7-Mb fragments of chromosome 5) that spans the entire chromosome in minimally overlapping segments.
- Isolation of about 100 cosmids from each of 30 bins defined by the final radiation hybrid contig.
- Isolation and sequencing of about 30 exons from each of the same 30 bins via exon amplification of pooled cosmids.
MAJOR ACCOMPLISHMENTS
- Placement of 1200 loci on chromosome 5 radiation hybrid map. Order of 850 loci was established with odds >10,000:1.
- Identification of 7 single-fragment radiation hybrids with different, defined segments of 5q; total coverage of 5q is about 30%.
- Establishment of complete cosmid contig spanning about 3 Mb in 5q31-32.
- Isolation of the hyperekplexia and achondroplasia genes.
AVAILABLE RESOURCES
- Natural deletion hybrids of chromosome 5.
- Selected radiation hybrids with defined 5 to 10 Mb of 5q. (Entire set will become available when the complete RH "contig" is complete.).
- Set of about 300 cosmids for fluorescence in situ hybridization, distributed relatively evenly along the chromosome.
- Placement of markers (short tandem repeat polymorphisms or genes) from other laboratories on the RH map with data being maintained confidentially for 3 months..
HGMIS staff
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).
