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Human Genome News Archive Edition

Human Genome News, Nov. 1994; 6(4):12

Washington University School of Medicine

(NIH, established 1990)
Bernard H. Brownstein, Assistant Director
CONTACT: Brownstein (314/362-3613 or -1199, Fax: -3203,; Center for Genetics in Medicine; 4566 Scott Ave., Box 8232; St. Louis, MO 63110.
Frank Burough
Ellson Chen (Applied Biosystems Division, Perkin-Elmer)
Terry Featherstone
Pui Kwok
Ramaiah Nagaraja
Volker Nowotny
John Rice
David States


  • Construction of sequence tagged site-based integrated physical and genetic maps of the X chromosome.
  • Implementation of megabase-level sequencing of selected X chromosome regions.


  • Completion (with collaborators) of yeast artificial chromosome coverage of a number of loci, including 2 Mb in the Huntington's disease region and 4 Mb in the major histocompatibility complex.
  • Formulation, testing, and implementation of sequence tagged site-content mapping.
  • Assembly of contigs >65% of chromosome 7 from materials that include >147 sequence tagged sites [of which 100 are highly polymorphic linkage probes obtained originally from Jean Weissenbach (Institut Pasteur) and Ray White (Univ. of Utah)] and a set of >5400 chromosome 7-specific yeast artificial chromosomes that are low in cocloning.
  • Assembly of over 85% of the X chromosome in contigs using 1400 sequence tagged sites and 5000 X-specific yeast artificial chromosomes. The contigs range up to 21 Mb in length, with fully rationalized maps available for Xq26-qter. Large contigs are now being aligned in Xp, Xq13, and Xq24-q26; 200 highly polymorphic gene-specific markers are being placed along the chromosome with cognate yeast artificial chromosomes.
  • Sequence tagged site and yeast artificial chromosome information has been submitted to Genome Data Base.
  • Wide distribution of Wash. Univ. human yeast artificial chromosome library. Submission to American Type Culture Collection for worldwide distribution of all X chromosome-specific yeast artificial chromosomes, including an X-specific yeast artificial chromosome library.
  • Assembly of robot-assisted workstation to screen yeast artificial chromosome libraries; includes capacity for up to 1800 polymerase chain reaction reactions/d.
  • Development of algorithms and software for analysis of sequence tagged site-content and radiation hybrid mapping data and for choosing polymerase chain reaction primers.
  • Design and construction of a database for sequence tagged site-content mapping data.
  • Implementation of SEGMAP for visual map representation and for testing sequence tagged site-based DNA segment contigs.


  • Assorted genomic and X-specific yeast artificial chromosome libraries (made at Wash. Univ. and elsewhere); all configured for polymerase chain reaction-screening yeast artificial chromosomes with sequence tagged sites.
  • Implemented rapid polymerase chain reaction screening of X-specific cosmid libraries.
  • High-throughput polymerase chain reaction machine.
  • Information on polymerase chain reaction conditions and end-cloning protocols.
  • UNIX-based software to run a Zymark side-loader arm in conjunction with a Biomek 1000 robot for robot-assisted screening, and information for preparing large libraries for polymerase chain reaction screening.
  • SEGMAP (yeast artificial chromosome contig assembly software).
  • Reliable procedures to recover yeast artificial chromosome insert ends and sequence them automatically.
  • Software for sequence tagged site development and data storage.

  • HGMIS staff

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Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

Human Genome News

Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.