Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, Nov. 1994; 6(4):10
Univerisity of Wisconsin
Madison E. Coli Genome Center
- UNIVERSITY OF WISCONSIN, MADISON (UWM) E.COLI GENOME CENTER
- (NIH, established 1991)
- FREDERICK R. BLATTNER, Director
- CONTACT: Blattner (608/262-2534, Fax: /263-7459, firstname.lastname@example.org); UWM; 445 Henry Mall; Madison, WI 53706.
- OTHER KEY RESEARCHERS
- David Argentar
- Valerie D. Burland
- Guy Plunkett III
- Debra Rose
- Pat Wathen
- Determination of the complete genome sequence of E. coli K12 strain MG1655 (4.7 Mb).
- Determination of the sequences of selected E. coli phages.
- Identification and annotation of genes and features of E. coli and its phages. Comparison and confirmation of all data with available published sequence to provide a coherent, consistent view.
- Distribution of results to research community.
- Development of improvements in sequencing technology and costs.
- Cooperation and coordination with other E. coli genome projects.
- Establishment of a production sequencing facility capable of producing 1 Mb/yr, with accuracy in the range of less than one error in 10 to the 5th nucleotides.
- Placement in GenBank of 1,061,766 bp of complete, contiguous, fully annotated E. coli sequence. (Largest contiguous segment of E. coli data in GenBank is now 1,244,699 bp, which includes Wisconsin and Japanese submissions.)
- Sequencing of 116,000 bp of phages phi80 and 933W in progress; provisional data available on ftp site.
- Development and application of technical innovations in robotics and sequencing strategies.
- Establishment of an ftp site (ecoliftp.genetics.wisc.edu).
- Full-coverage ordered clone bank of E. coli MG1655 in lambda vectors; Janus M13 "flipping" vector.
- Sequencing production laboratory (5000 sq. ft.) equipped with Applied Biosystems Inc., Li-Cor and radioactive sequencing technology.
- High-speed network of UNIX workstations and Macintosh and IBM-compatible computers. Condor parallel processing system allows multiple workstations to solve a single problem.
- Extensive custom and commercial software for sequence acquisition and analysis, including assembly, gene detection, annotation, and Informix-based DNA sequence production management.
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).