Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, March 1993; 4(6)
GENOME CENTER:
Washington University School of Medicine
(NIH; established 1990)
DIRECTOR:
David Schlessinger
CONTACT:
David Schlessinger (314/362-1188, Fax: -3208)
Washington University School of Medicine
Center for Genetics in Medicine
4566 Scott Avenue, Box 8232
St. Louis, MO 63110
OTHER KEY RESEARCHERS:
Buddy Brownstein, David Chaplin, Eric Green, Phil Green, Volker Nowotny
MAJOR GOALS:
- Construction of integrated physical and genetic maps of the X chromosome and chromosome 7 and targeted mapping of several megabase regions elsewhere in the human genome.
MAJOR ACCOMPLISHMENTS:
- Completion (with collaborators) of the YAC coverage of a number of loci, including 2 Mb in the Huntington's region and 4 Mb in the major histocompatibility complex.
- Assembly of chromosome 7 materials that include > 700 STSs [of which 100 are highly polymorphic linkage probes obtained originally from Jean Weissenbach (Institut Pasteur) and Ray White (University of Utah)] and a set of over 2500 chromosome 7þspecific YACs that are low in cocloning.
- Assembly of over 40% of the X chromosome in contigs ranging up to 9 Mb in length, with rationalized maps available for Xq26-qter. Large contigs are now being aligned in Xp, Xq13, and Xq24-q26, and 60 highly polymorphic or gene-specific markers placed along the chromosome with cognate YACs.
- Wide distribution of Washington Univ. human YAC library.
- Assembly of robot-assisted workstation to screen YAC libraries, including the capacity for up to 1800 PCR reactions per day.
- Formulation and initial testing of STS-content mapping.
- Development of algorithms and software for analysis of STS-content and radiation hybrid mapping data, and for choosing PCR primers.
- Design and construction of a database for STS-content mapping data.
AVAILABLE RESOURCES:
- High-throughput PCR machine.
- Information on PCR conditions and end-cloning protocols.
- Software to run a Zymark side-loader arm in conjunction with a Biomek robot for robot-assisted screening.
- SEGMAP (YAC contig assembly software).
- Reliable procedures to recover YAC insert ends and sequence them automatically.
- Software for STS development and data storage.
Genome Centers Acronym List
HGMIS Staff
Return to List of Genome Centers
Return to Table of Contents
The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v4n6).
