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Human Genome News, January 1998; 9:(1-2)

Table 1.  Large DNA Cloning Systems

Host Yeast Bacteria Bacteria Human Mouse
Genetic Elements ARS/CEN F episome P1 bacteriophage OriP/EBNA-1 HAEC OriP/EBNA-1
DNA Status Linear Circular Circular Circular Circular
Insert size (kb) 100 to 2000 40 to 300 40 to 250 80 to 350 80 to 120
Copy#/cell 1 1 1 ~1 to 10 ~1 to 10
Amplification - - 20x 1000x (+)
Viral Stock - - + + (ND*)
Non-Recombinant - + + - (ND)
Chimeras + - - - (ND)
Stability (+ -) + + + (+)
Major application STS linkage, contig maps Contig maps, sequencing Contig maps, sequencing Functional analysis Functional analysis
References Burke et al. (1987) Shizuya et al. (1992) loannou et al. (1994) Sun et al. (1994) Kelleher et al. (1997)

 * not determined.

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