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Human Genome News, January 1998; 9:(1-2)
YAC | BAC | PAC | HAEC | MAEC | |
---|---|---|---|---|---|
Host | Yeast | Bacteria | Bacteria | Human | Mouse |
Genetic Elements | ARS/CEN | F episome | P1 bacteriophage | OriP/EBNA-1 | HAEC OriP/EBNA-1 |
DNA Status | Linear | Circular | Circular | Circular | Circular |
Insert size (kb) | 100 to 2000 | 40 to 300 | 40 to 250 | 80 to 350 | 80 to 120 |
Copy#/cell | 1 | 1 | 1 | ~1 to 10 | ~1 to 10 |
Amplification | - | - | 20x | 1000x | (+) |
Viral Stock | - | - | + | + | (ND*) |
Non-Recombinant | - | + | + | - | (ND) |
Chimeras | + | - | - | - | (ND) |
Stability | (+ -) | + | + | + | (+) |
Major application | STS linkage, contig maps | Contig maps, sequencing | Contig maps, sequencing | Functional analysis | Functional analysis |
References | Burke et al. (1987) | Shizuya et al. (1992) | loannou et al. (1994) | Sun et al. (1994) | Kelleher et al. (1997) |
* not determined.
Back to "Large-Insert Cloning Vectors Aid Function Studies"
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Human Genome Program, U.S. Department of Energy, Human Genome News (v9n1).
The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.
Published from 1989 until 2002, this newsletter facilitated HGP communication, helped prevent duplication of research effort, and informed persons interested in genome research.