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Dear Human Genome Researchers,

Our laboratory has developed strategies to bridge contig gaps occurring at human genomic regions which cannot be cloned and/or maintained faithfully in bacteria using the large cloning systems (PI/BAC/PAC,etc). Such strategies derive from the original HAEC technology which allows direct cloning of DNA in human cells as Human Artificial Episomal Chromosomes (Nature Genetics 8:33-41, 1994; Methods in Molecular Genetics 8:167-188, 1996).

We have now entered the phase of testing such technology for proof-of-concept. We will provide the human genome community with a resource to isolate and sequence refractory contig gaps. Hence, we are interested in hearing from the human genome community about persistent and refractory genomic gaps in order to identify potential candidates for the HAEC bridging technology. Suitable candidates are contig gaps which have been thoroughly tested on highly redundant large bacterial and yeast libraries and also shown to be missing in libraries prepared with different restriction enzymes.

Interested human genome laboratories should send their email reply to the attention of Jean-Michel Vos at the following address: angemari@gibbs.oit.unc.edu.

Dr. Jean-Michel H. Vos
349 Lineberger Comprehensive Cancer Center
School of Medicine CB#7295
University of North Carolina at Chapel Hill
Chapel Hill, NC  27599-7295
Fax:  919-966-3015
Vos lab web site:  http://www.med.unc.edu/wrkunits/3ctrpgm/lccc/voslab

This HAEC research is sponsored by the Department of Energy Human Genome Program.

Last modified: Wednesday, October 22, 2003

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