BeBeryllium Research Symposium: Basic Mechanisms and Human Health
June 25- 26, 2002
National Library of Medicine, Bethesda, MD





 Session 1

 Session 2

 Session 3

 Session 4

 Session 5


Sponsored by
Department of Energy Seal
Office of Biological and Environmental Research,
The Department of Energy
in cooperation with


The National Institute for Occupational Safety and Health, and

National Jewish Medical and Research Center
The National Jewish Medical and Research Center



8 - 8:30 Registration
8:30 - 8:40 Welcome from DOE
Susan Rose
8:40 - 8:50 Welcome on Behalf of the Planning Committee
Sally Tinkle
8:50- 9:35 Multi-Disciplinary Research-the Key to Solving the Beryllium Disease Puzzle
Kathleen Kreiss

Session 1. Immunopathology of Beryllium-Induced Granulomatous Disease
Session chair: Brian Kotzin

9:35 - 10:15 Immunopathogenesis of Sarcoidosis
Gianpietro Semanzato
10:15 - 10:30 Break
10:35 -10:55 Characterization of Beryllium-Specific Memory CD4+ T Cells in Patients with Chronic Beryllium Disease
Andrew P. Fontenot, Scott J. Canavera, Laia Gharavi, Lee S. Newman, and Brian L. Kotzin
10:55 - 11:15 HLA-DP-Unrestricted TNF-a Release in Be-Stimulated PBMC from Berylliosis Patients
Massimo Amicosante, Floriana Berretta, Alberto Franchi, Paola Rogliani, Chiara Dotti, Monica Losi, Raed Dweik, and Cesare Saltini
11:15 - 11:35 Design, Engineering and Production of Human Recombinant T Cell Receptor Ligands Derived from HLA-DP
Gregory G. Burrows, Roberto Meza-Romero, Jeff Mooney, Justin W. Chang, Hans Peter Bachinger, and Jianya Huan
11:35 - 11:55 A Flow Cytometric Assay for Beryllium Sensitization: Screening and Mechanistic Applications
A.J. Jabbour, R.A. Ponce, M.T. Rosato, T.J. Kavanagh, L.S. Newman, T.K. Takaro, and E.M. Faustman
12:00 - 1:00 Lunch

Session 2. Pulmonary Signaling Pathways Activated by Beryllium
Session chair: Lisa Maier
1:00 - 1:45 Second Messenger Pathways Activated by Endotoxin and Asbestosis in Alveolar Macrophages
Gary Hunninghake
1:45- 2:05 Beryllium Stimulates a Local Angiotensin System in Chronic Beryllium Disease
T. Hendry-Hofer, A.F. Fontenot, E.A. Barker, M. Boguniewicz, L.S. Newman, and L.A. Maier
2:05 - 2:25 High Levels of Nitric Oxide (NO) in Exhaled Breath in Patients with Chronic Beryllium Disease
R.A. Dweik, D. Laskowski, and S.C. Erzurum
2:25 - 2:45 Nitric Oxide (NO) Attenuation of Interferon Gamma (IFN-Gamma) Responses in Chronic Beryllium Disease (CBD): Evidence for Mechanisms Independent of Interleukin (IL)-18
M.J. Thomassen, C. Farver, R.A. Dweik, D. Culver, B. Yen-Lieberman, M. Kavuru, and B.P. Barna
2:45 - 3:00 Break

Session 3. Physico-Chemical Properties of Beryllium Metal and Alloys
Session chair: Babetta Marrone
3:00 - 3:45 Physicochemical Determinants of Beryllium Toxicity Using In Vitro and In Vivo Models
Greg Finch
3:45 - 4:05 Dosimetry of Beryllium in an Animal Model by Accelerator Mass Spectrometry
M.L. Chiarappa-Zucca, R.C. Finkel, J.E. McAninch, R.E. Martinelli, and K.W.Turteltaub
4:05 - 4:25 Identification of Non-Toxic Beryllium-Chelating Porphyrins and Method of Delivery to Mice in an Attempt to Reduce the Lung Beryllium (Be) Burden in Chronic Beryllium Disease (CBD)
Jim Klostergaard and George P. Sakalosky
4:25 - 4:45 Surface Area of Respirable Beryllium Metal, Oxide, and Copper Alloy Aerosols and Implications for Assessment of Exposure Risk of Chronic Beryllium Disease
A.B. Stefaniak, M.D. Hoover, R.M. Dickerson, E.J. Peterson, G.A. Day, P.N. Breysse, M.S. Kent, and R.C. Scripsick
4:45 - 5:05 Examining Beryllium Chemistry with Modern Analytical Techniques
M. Sutton, S.R. Burastero, C. Mundy, and J. Quong
5:05 Social gathering-location to be announced

Wednesday, June 26, 2002

Session 4. Gene - Exposure Interactions
Session chair: Cesare Saltini

9 - 9:45 Chemicals + Immunologic Lung Disease: Gene + Environment
Anthony Newman-Taylor
9:45 - 10:05 Functional TNF-a Promoter Polymorphisms Associated with Chronic Beryllium Disease and Beryllium-Induced TNF-a
L.A. Maier, R.T. Sawyer, T. Hendry-Hofer, C. Parsons, R. Bauer, D. McGrath, P. Lympany, R. duBois, and L.S. Newman
10:05 - 10:25 Association of HLA Class II Markers and Beryllium Hypersensitivity and Disease: Reevaluation of Previously Published Data After 4-8 Years of Follow-up
C. Saltini and M. Rossman
10:25 - 10:45 Electrostatic Potential on HLA-DP*1701 and HLA-DP*0401: Implications for Putative Mechanism of Chronic Beryllium Disease
James A. Snyder, Ainsley Weston, Sally T. Tinkle, and Eugene Demchuk
10:45 - 11:00 Break

Session 5. New Directions in Pulmonary Research
Session chair: Milton Rossman

11:00 - 11:45 Effects and Fate of Ultrafine Particles
Günter Oberdörster
11:45 - 1:00 Lunch
1:00 - 1:20 Cutaneous Application of Beryllium Salts and Oxide Particles Produces Peripheral Sensitization in the C3H/HeOuJ Mice
Sally S. Tinkle, James M. Antonini, Jenny R. Roberts, Rebecca Salmen, Karyn Depree, and Melanie Flint

1:20 - 1:40

In Vitro Transformation of Phagocytized Beryllium Oxide Particles in the Murine J774A.1 Cell
G.A. Day, A.B. Stefaniak, M.D. Hoover, R.M. Dickerson, E.J. Peterson, N.A. Esmen, and R.C. Scripsick
1:40 - 2:00 The Mouse Deep Lung is Exposed to Respirable Particles Administered by Pharyngeal Aspiration
A.F. Hubbs, G.V.S. Rao, M. Kashon, R. Salmen, L.A. Battelli, P. Willard, J. Antonini, D.N. Weissman, and S. Tinkle
2:00 - 2:20 Beryllium-Induced Macrophage Apoptosis in Chronic Beryllium Disease
R.T. Sawyer, V.A. Fadok, L.A. Maier, L.A. Kittle, J.M. Routes, and L.S. Newman
2:20 - 2:35 Break
2:35 - 3:35 Ethical, Legal and Social Implications of Beryllium Exposure and Research: Case Presentation
Panel: Reed Durham, beryllium chemist; Myron Harrison, occupational medicine; Jon Moreno, bioethicist; Susan Rose, bioethicist
3:35 - 3:55 Meeting Overview - Lee Newman
3:55 - 4:00 Closing Remarks - Sally Tinkle


Original Announcements: Agenda - Symposium Information - Speakers - Symposia - Hotel Accommodations - Registration Form - Abstract Submission Form - Poster (pdf, 1365 KB)