A 1500 Markers Radiation Hybrid Map of the Dog Genome

Catherine Andre
CNRS UPR41 "Recombinaisons Génétiques"
Faculté de médecine
2, Avenue du Pr. Leon Bernard
35043 Rennes cedex FRANCE
telephone: 33 (0) 2 99 33 62 47
fax: 33 (0) 2 99 33 62 00
email: candre@univ-rennes1.fr
prestype: Platform
presenter: Catherine Andre

Catherine André, Angélique Chéron, Christophe Hitte, Zhihua Jiang, Sophie Jouquand, Catherine Priat, Corinne Renier, Françoise Vignaux & Francis Galibert
UPR41 CNRS, Recombinaisons Génétiques, Faculté de Médecine, 2 avenue du Pr. Léon Bernard, 35043 Rennes cedex, France.
Tel : 33 (0) 2 99 33 62 47 Fax : 33 (0) 2 99 33 62 00
E.mail : candre@univ-rennes1.fr.

Dog as a species stands out as a powerful genetic model, as the 300 well-established breeds harbour remarkable interbreed heterogeneity contrasting with strong intrabreed uniformity. This particular feature makes dog an invaluable resource for dissecting the molecular basis of genetic traits and diseases.

To this end we have constructed a whole genome dog /hamster radiation hybrid panel: RHDF5000 (Vignaux et al., 1999). In addition, 300 dog gene markers and 1000 microsatellite markers have been identified (Priat et al., 1999; Jouquand et al., 2000). With these tools we constructed a radiation hybrid map of the dog (i) by positioning 400 markers (Priat et al.,1998) and (ii) by integrating through a collaborative efforts (Mellersh et al., 2000), the Radiation hybrid map containing 600 markers into the Linkage map. At the present time, each of the 39 canine chromosomes (CFA1 to 38 and X and Y) has been assigned to the corresponding radiation hybrid group (Breen et al., in preparation) and a 1500-markers radiation hybrid map has recently been achieved (Jouquand et al., in preparation). This map, harbouring 1200 microsatellites and 300 genes covers 95% of the dog genome and allowed us to characterize conserved segments between the canine and human chromosomes. Using this map we plan to identify diseases or phenotypic /behavioural traits by linking the trait with polymorphic markers using the candidate gene approach.

Data available on: http://www-recomgen.univ-rennes1.fr/doggy.html.

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