Gene Trapping Identified Transiently Induced Survival Genes During Programmed Cell Death

Harald von Melchner
Laboratory for Molecular Hematology
University of Frankfurt Medical School
60590 Frankfurt am Main, Germany
telephone: +49-69-63016696
fax: +49-69-63016390
prestype: Platform
presenter: Frank Wempe

Frank Wempe, Ji-Yeon Yang, and Harald von Melchner
Laboratory for Molecular Hematology, University of Frankfurt Medical School, Frankfurt am Main, Germany

One of the most common forms of physiologic apoptosis occurs as aconsequence of "survival" factor deprivation. To identify genes that are transiently induced during this process, we have used a strategy based on gene trap mutagenesis and site specific recombination (Cre/ loxP). We show here that hematopoietic cells undergoing apoptosis by growth factor (IL3) deprivation upregulate survival genes prior to irreversible death commitment. The activation of protective mechanisms during the early stage of programmed cells death results in reduced apoptosis and improved survival of cells treated with a transient apoptotic stimulus. Thus, we conclude that apoptosis in hematopoietic cells is the end result of a conflict between death and survival signals, rather than a simple death by default.

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