Planarian Gene Expression Profiles Using cDNA Chip Technology

National Institute of Genetics
Center for Information of Biology
Yata1111, Mishima-city, Shizuoka pref.
411-8540 Japan
telephone: +81-559-81-6847
fax: +81-559-81-6848
prestype: Poster
presenter: Masumi NAKAZAWA

Masumi NAKAZAWA1, Katsuhiko MINETA1, Kazuho IKEO1, Kiyokazu AGATA2, Takashi GOJOBORI1
1National Institute of Genetics, 2Okayama University, Japan

The primitive brain equipped animal, planarian (Platyhelminthes) gives us good evolutionary insights for brain study. We made a planarian cDNA microarray including 1570 genes, following our planarian EST project (Dugesia japonica) that has produced about 9000 sequences (Mineta et al, In preparation). One of neuron specific genes, Synaptotagmin (Djsyt) was found out from our ESTs and further study was reported (Tazaki et al. BBRC 260: 426-432, 1999). Our planarian cDNA chip is containing 17 neuron related genes, 13 transcription factors, 65 protein kinases, 270 hypothetical protein coding genes, 18 unknown genes and so on. This cDNA chip is used to detect brain specific genes doing competitive hybridization between mRNAs derived from head and body part of planarians. The current result shows planarian brain specific genes contain not only human neural gene homologues but also unknown genes, which seem planarian specific genes. We also attempt RNA interference technique to make brain-specific genes knocked out planarian. We will report the planarian brain specific genes and its expression profiles during regeneration and on knocked out planarian.

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