Beyond the Identification of Transcribed Sequences:
Functional, Evolutionary and Expression Analysis
12th International Workshop
October 25-28, 2002
Washington, DC

List of Abstracts * Speakers * Organizers * Authors * Original Announcement

Resources for Functional Research: Repository and Application

Bernhard Korn
RZPD, Heidelberg, 69120 GERMANY
Telephone: +49 (0)6221 42 4700
Fax: +49 (0)6221 42 4704

With the multinational Human Genome Project, molecular biology and medicine entered large scale and high throughput approaches and applications. A new era of technology development, information collection and sharing of material was started. One of the most challenging next steps will be the understanding of the functional roles for all proteins. RZPD has started to establish a sequence-verified collection of full open reading frames (ORF) cDNAs representing more than 3.000 human genes. A shuttle vector system is used that allows protein expression in a wide range of hosts as well as in vivo and in vitro functional studies at different scales. In order to find out more about the influence of domains and secondary structure to the ability to express proteins in different systems, we perform highly parallel expression of ORFs in vitro and in vivo. In addition, the proteins are used in a matrix Two-Hybrid approach to gain more insights into human protein networks. The identification of interaction partners will be valuable to assign potential functions to proteins that are not characterised otherwise yet. RZPD is seeking to provide genomic and proteomic resources to the community. Beside genomic and cDNA clones and arrays, the development of material that is capable to directly support functional studies will be established, controlled and distributed.

  Abstract List

List of Abstracts * Speakers * Organizers * Authors * Original Announcement

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