Beyond the Identification of Transcribed Sequences:
Functional, Evolutionary and Expression Analysis
12th International Workshop
October 25-28, 2002
Washington, DC

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Crucial Elements in Array Data Evaluation

Michael Liebler and Till Sörensen
raytest GmbH, Aichtal, D 72631 GERMANY
Telephone: +49 7082 9255 0
Fax: +49 7082 20813

Gene profiling in array technology is a quantitative method, in which signal detection results in grayscale image-data. The following data processing with evaluation softwares produces large amounts of result-data. Quality and reproducability of these data depends strongly on some essential steps of  the computing process like spot alignment and background subtraction. The actual existing evaluation softwares for array experiments use different strategies for these evaluation steps. Investigations of these different evaluation strategies on slide or membrane shown, that these crucial elements have to be adepted to the various array designs. Possibilities for a quality control in form of spot rating will be  discussed.

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