Beyond the Identification of Transcribed Sequences: Functional and Expression Analysis

9th Annual Workshop, October 28-31, 1999

Co-sponsored by the U.S. Department of Energy


Comparative mapping in the Japanese pufferfish (Fugu rubripes)

Clark, M. S; Edwards, Y.J.K; Shaw, L; Snell, P.; Smith, S; and Elgar, G.

Fugu Genomics HGMP Resource Centre, Wellcome Genome Campus, Hinxton, Cambridge, United Kingdom

The project within this laboratory to randomly sequence scan the Fugu genome finished a year ago. Since then we have concentrated on analysing three regions in depth, equivalent to the human chromosomal regions 11p, 20q and MHC. These regions are currently being mapped and, to a limited extent, sequenced. Data currently available indicates the utility of the Fugu genome for identifying conserved regulatory elements, novel genes, confirming predicted genes in human and evolutionary conservation of gene blocks. Examples of these will be illustrated from current unpublished research. There is also an ongoing project to develop ESTs in Fugu. A number of cDNA libraries have been constructed and so far 3,000 sequences have been randomly sequenced from these. Clustered data will be presented on these sequences.

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