Beyond the Identification of Transcribed Sequences: Functional and Expression Analysis

9th Annual Workshop, October 28-31, 1999

Co-sponsored by the U.S. Department of Energy


Sequencing and analysis of full length cDNAs in the course of the German Genome Project

Stefan Wiemann1, Wilhelm Ansorge, Helmut Blöcker, Helmut Blum, Andreas Düsterhöft, Karl Köhrer, Werner Mewes, Brigitte Obermaier, Rolf Wambutt, and Annemarie Poustka

1Molecular Genome Analysis, German Cancer Research Center and the German cDNA Sequencing Consortium, Heidelberg, Germany

A consortium of eight sequencing laboratories and Germany's leading bioinformatics institute has formed in the frame of the German Genome Project. We aim at the sequence analysis of 3,000 to 4,000 complete novel cDNAs, comprising eight megabases of finished sequence. Sequencing started in September 1997 and a progress report of the consortium will be presented. The libraries generated in the course of the grant "Generation of full length cDNAs in the course of the German Genome Project" are the primary source for sequencing. EST sequences of 12,000 independent clones are generated to identify novel genes. The EST sequences are analyzed for the likelihood of the clones to be full length (e.g. by the presence of CpG clusters) in order to obtain a minimal set of full length clones for efficient complete sequence analysis. Clones identified to be full length are sequenced and further analyzed by members of the consortium. The sequences are analyzed for possible function in silico. Functional analysis projects have started using the clones analyzed by the consortium as resource. All clones and data generated in the project are made publicly available via the Resource Centre of the German Genome Project (RZPD).


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