TRANSCRIPTOME 2002: From Functional Genomics to Systems Biology
March 10-13, 2002
Seattle, Washington, USA


Expression of Cytokines and Growth Factors in Aging and hTERT-transfected Human Fibroblasts

Yukari Kanzaki, Toshinori Ide, Hiroshima University School of Medicine, Hiroshima, JAPAN

Telomerase is the unique reverse transcriptase that synthesizes telomeric DNA onto chromosomal ends using its RNA component as a template. Telomerase expressed in the most cancer cells, but not somatic cells except germline cells and stem cells. We surveyed, using membrane array hybridization, the expression of some growth factors and cytokines in human fibroblasts transfected with human telomerase reverse transcriptase subunit (hTERT) cDNA. Twelve genes out of 268 were induced and 7 out of 268 were repressed in hTERT-transfected fibroblasts. Telomerase are specialized structure at the end of eukaryotic chromosomes and concerned with chromosomal stability. Telomere DNA shortens by 50-200 b.p. per cell division and telomere length has been proposed as a counting mechanism of cell division number that controls cellular senescence. We have examined the changes in gene expression in accordance with aging in vitro. Moreover we examined whether telomere elongation rescues expressional changes, which occur during aging.

Return to Table of Contents * Speaker Abstracts * Poster Abstracts * View the Photos

Return to Meetings Home Page

This site produced by the Human Genome Management Information System of Oak Ridge National Laboratory.