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Human Genome News Archive Edition

Human Genome News, September 1990; 2(3)

Workshop: Mapping Human Chromosome 22: Progress and Strategies

An international group of 34 participants gathered in Paris under the sponsorship of the Ministere de la Recherche et de la Technologie (France) and the NIH National Center for Human Genome Research (United States) April 27-30 for a workshop on "Mapping of Human Chromosome 22: Progress and Strategies."

The first session covered recent observations on the involvement of chromosome 22 in neoplasia. The second session was devoted to describing translocations of chromosome 22 and hybrid mapping panels. The presentations highlighted the usefulness of these cell lines for regional localization of the rapidly growing number of new DNA markers.

A new chromosome-22-only hybrid (GM10888) that appears to be free of other human chromosomes has been banked at the Human Mutant Cell Repository of Coriell Institute in Camden, New Jersey. Many investigators have been using other hybrid cell lines as starting material for chromosome-22-specific libraries, as well as radiation hybrids of those lines. Screening and mapping efforts with these lines may be complicated by additional material that originated from chromosomes other than 22.

Significant attention was devoted to physical mapping technologies and their application to mapping chromosome 22:

  • yeast artificial chromosome (YAC) and cosmid approaches to chromosomal mapping; microcloning of chromosome-22-specific libraries;
  • radiation hybrid and long-range mapping approaches to chromosome 22; and
  • development of a new large-fragment cloning vector, the bacterial artificial chromosome (BAC), which is based on an F factor modified to contain a COS site, a selectable marker, polymerase initiation sites, and cloning sites.

The most recent genetic map of 22q was reported to use 17 markers and cover 102 cM from the most proximal marker (D22S24) to the most telomeric of the mapped markers on 22q (D22S45). A separate session dealt with mapping the regions of chromosome 22 that are involved in DiGeorge and Cat Eye syndromes.

Participants agreed that yearly workshops would be desirable to maximize sharing of data and materials. The group selected a set of 15 reference markers for chromosome 22, 10 of which were designated as consensus anchor markers, and identified a set of 4 nonrandom translocation breakpoints as anchors in the map. A set of standard hybrid cell lines was designated for use in mapping chromosome 22; these hybrids will be banked at the Human Mutant Cell Repository.

For more information, contact:

  • Beverly Emanuel
    The Children's Hospital of Philadelphia
    34th St. and Civic Blvd.
    Philadelphia, PA 19104
    (215) 590-3855
    Fax: (215) 590-3850

Reported by Beverly S. Emanuel

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Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.

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