Human Genome News, March 1993; 4(6)

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Human Genome News, March 1993; 4(6)

GENOME CENTER:

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology

(NIH; established 1990)

DIRECTORS:
Eric S. Lander
David Page and Nat Goodman, Associate Directors

CONTACT:
Eric Lander (617/258-5192, Fax: -6505; lander@mitwibr.bitnet)
Whitehead Institute for Biomedical Research
Nine Cambridge Center
Cambridge, MA 02142

OTHER KEY RESEARCHERS:
Daniel Cohen (CEPH), Nic Dracopoli (Massachusetts Institute of Technology), Rudolf Jaenisch (Whitehead Institute for Biomedical Research), Joe Nadeau (Jackson Laboratory), Shirley Tilghman (Princeton University, HHMI)

MAJOR GOALS:

Construction (in 3 years) of a low-resolution physical map of the human genome based on 8500 STSs.
Construction of genetic and physical maps of the mouse genome, including a high-resolution genetic map consisting of 6000 SSLPs, integrating 1/4 of these markers with the Copeland/Jenkins cross showing the location of the genes. Production (within 5 years) of a low-resolution physical contig map (average size, 10 to 20 Mb) based on 10,000 STSs.

MAJOR ACCOMPLISHMENTS:

Construction of a map having > 1000 SSLPs.
Construction of a mouse genome YAC library with 700-kb average inserts.
Implementation of an efficient approach for screening entire YAC libraries.
Implementation of a center-pioneered object-oriented database for genomic data.
Introduction of YACs into mouse ES cell lines and into the mouse germline.
Development of new technologies to increase automation ("waffle iron" thermocycler, which can handle 16 microtitre dishes at once), STS screening robot.

AVAILABLE RESOURCES:

Mouse genome YAC library.
Primers for mouse SSLPs (available from Research Genetics, Inc., Huntsville, AL).
Software for choosing primers and genetic mapping.
"Waffle iron" thermocycler prototype (Automated Systems, Cambridge, MA).
CEPH Mark II to VII (inserts, 400 kb to > 1 Mb) YAC library.

Genome Centers Acronym List

HGMIS Staff

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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v4n6).

Human Genome Project 1990–2003

The Human Genome Project (HGP) was an international 13-year effort, 1990 to 2003. Primary goals were to discover the complete set of human genes and make them accessible for further biological study, and determine the complete sequence of DNA bases in the human genome. See Timeline for more HGP history.