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Human Genome News Archive Edition

Human Genome News, Nov. 1994; 6(4):8

University of California, Irvine

UNIVERSITY OF CALIFORNIA, IRVINE (UCI)
(NIH, established 1993)
JOHN J. WASMUTH, Director
John D. McPherson, Co-Director
CONTACTS: Wasmuth (714/856-7067), McPherson (-8242), or Judy Brown, Administrative Assistant (-7067); (Center Fax: /725-3403); UCI; Natl. Hum. Gen. Res. Center; Coll. of Med.; Irvine, CA 92717.

MAJOR GOALS

  • Construction of 150-kb-resolution radiation hybrid map of human chromosome 5 including all highly polymorphic short tandem repeat polymorphism markers and every known gene on the chromosome.
  • Establishment of a radiation hybrid contig (from single 5- to 7-Mb fragments of chromosome 5) that spans the entire chromosome in minimally overlapping segments.
  • Isolation of about 100 cosmids from each of 30 bins defined by the final radiation hybrid contig.
  • Isolation and sequencing of about 30 exons from each of the same 30 bins via exon amplification of pooled cosmids.

MAJOR ACCOMPLISHMENTS

  • Placement of 1200 loci on chromosome 5 radiation hybrid map. Order of 850 loci was established with odds >10,000:1.
  • Identification of 7 single-fragment radiation hybrids with different, defined segments of 5q; total coverage of 5q is about 30%.
  • Establishment of complete cosmid contig spanning about 3 Mb in 5q31-32.
  • Isolation of the hyperekplexia and achondroplasia genes.

AVAILABLE RESOURCES

  • Natural deletion hybrids of chromosome 5.
  • Selected radiation hybrids with defined 5 to 10 Mb of 5q. (Entire set will become available when the complete RH "contig" is complete.).
  • Set of about 300 cosmids for fluorescence in situ hybridization, distributed relatively evenly along the chromosome.
  • Placement of markers (short tandem repeat polymorphisms or genes) from other laboratories on the RH map with data being maintained confidentially for 3 months..

HGMIS staff

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Human Genome Program, U.S. Department of Energy, Human Genome News (v6n4).

Human Genome Project 1990–2003

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