Sponsored by the U.S. Department of Energy Human Genome Program
Human Genome News, March 1993; 4(6)
Los Alamos National Laboratory
(DOE; established 1988)
Robert K. Moyzis
Larry L. Deaven, Deputy Director
Lynn Clark, Technical Coordinator
(505/667-9376, Fax: -2891; firstname.lastname@example.org)
Los Alamos National Laboratory
Center for Human Genome Studies
Life Sciences Division, MS M886
Los Alamos, NM 87545
OTHER KEY RESEARCHERS:
Michael Altherr,Tony Beugelsdijk, Michael Cinkosky, Norman Doggett, Jim Fickett, Deborah Grady, Ed Hildebrand, Dick Keller, Jon Longmire, Mary Kay McCormick, Pat Medvick, Julie Meyne, David Torney, Michael Yesley
- Assembly of complete high-resolution (0.1 Mb) maps of chromosome 16 and chromosome arm 5p.
- Determination of the molecular basis of chromosome structure and function and isolation of selected disease genes on chromosomes 5 and 16.
- Short-term development and support for large-scale physical mapping and sequencing projects and long-term development of tools for the storage, manipulation, and analysis of genome data.
- Development and application of newmethods for physical mapping; use of robotics in handling and storing DNA fragments; construction of DNA libraries from flow-sorted chromosomes; and rapid, inexpensive, large-scale sequencing.
- Studies of ethical, legal, and social issues arising from the increased availability of genome data.
- Construction of maps, including (1) a high-resolution cosmid/YAC physical map of human chromosome 16 consisting of 500 contigs covering over 95% of the chromosome (map translated into framework STS map of 150 new regionally localized markers) and (2) a framework STS map of human chromosome 5 [with John Wasmuth (UC, Irvine)] consisting of 200 new markers regionally assigned with a resolution of 4 Mb (5q) to 1 Mb (5p).
- Biological developments, including (1) identification and cloning of the human telomere, the endpoint for genetic and physical maps; (2) determination of unusual 3-D structure of telomeric DNA [with Alex Rich (MIT)]; (3) identification and cloning of highly conserved centromeric repetitive DNA regions, likely human centromere components; and (4) construction of a novel cDNA library from mRNA obtained from a paternally encoded human pregnancy (hydatidiform mole).
- Development of technology, including (1) NLGLP (with LLNL) chromosome-specific libraries (over 2500 DNA libraries sent to research and production laboratories world-wide, including complete digest libraries for each human chromosome; partial-digest phage and cosmid libraries for human chromosomes 4, 5, 6, 8, 10, 11, 13, 14, 16, 17, 20, X, and Y; and complete digest low-chimeric YAC libraries for human chromosomes 5, 9, 16, and 21; (2) flow-cytometry techniques to detect single DNA molecules, resulting in a CRADA with LTI for codevelopment of a rapid DNA-sequencing technology; and (3) a robot for high-density cosmid/YAC array replication and distribution.
- Phage and cosmid libraries developed within NLGLP (see accomplishments).
- Low-chimeric YAC libraries: total genomic (180 kb, average insert) and chromosome-specific for chromosomes 5, 9, 16, and 21.
- CEPH Mark II to VII YAC libraries.
- High-density filter arrays of cosmid and YAC libraries.
- STS collections for chromosomes 16 (200) and 5 (200).
- Panel of somatic cell hybrids for sorting each human chromosome.
- SIGMA, a graphical map editor.
- cDNA-inform database and software for comparison of sequences.
- Modified Biomek robot and LANL robot for high-density array construction.
Genome Centers Acronym List
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The electronic form of the newsletter may be cited in the following style:
Human Genome Program, U.S. Department of Energy, Human Genome News (v4n6).